| 乌江彭水水电站截流前后白甲鱼群体遗传结构初步研究 |
| Preliminary study on population genetic structure of Onychostoma sima before and after closure of Pengshui hydropower station on Wujiang River |
| 投稿时间：2012-11-30 修订日期：2012-12-12 |
| DOI： |
| 中文关键词:白甲鱼 遗传结构 微卫星 彭水水电站 |
| 英文关键词:Onychostoma sima genetic structure microsatellite Pengshui hydropower station |
| 基金项目:长江上游珍稀特有鱼类人工繁殖、放流技术试点示范（ＳＸ[2009]140)；科技部国际科技合作项目（2008DFA31550） |
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| 中文摘要: |
| 彭水水电站2008年1月下闸截流蓄水。以2006-2009年间采集的白甲鱼截流前群体、截流后坝上群体、截流后坝下群体为研究材料,利用白甲鱼微卫星遗传标记对其种群遗传结构进行分析。结果表明:由多态信息含量指数(PIC)和期望杂合度(He)检测的群体遗传多样性,截流后坝上群体的最高,截流前群体和截流后坝下群体接近;根据Nei’s遗传距离构建的UPGMA聚类图显示,截流前群体和截流后坝下群体先聚成一类,再与截流后坝上群体相聚。群体间Fst为0.0466,显示群体间遗传分化较弱,遗传变异较小。初步研究结果表明,截流前后的3个白甲鱼群体遗传多样性较丰富,群体间遗传变异较小。要准确完整地评估水电站对白甲鱼的遗传变异的影响,还需要长期的监测和进一步的分析。 |
| 英文摘要: |
| Fragmentation of the Wujiang River ecosystem by Pengshui hydropower station may disrupt the river connectivity and communication of fish populations，consequently，fish population genetic structure and diversity will be affected. In this study，thirteen Simple Sequence Repeat(SSR ) markers were used to compare genetic diversity among three Onychostoma sima populations which were consisted of before closure population of Pengshui hydropower station(BC)，above dam population after closure(AD)，and below dam population after closure(BD)from2006 to 2009. Expected heterozygosity(He)，observed heterozygosity(Ho)，polymorphic information content(PIC) and number of effective alleles(Ne) were all determined. The results showed that genetic diversity of AD population was highest，and the other two were lower. He and PIC value of AD population were highest with values of 0.6869 and 0.628. The genetic similarity coefficient and Nei's standard genetic distance were computed based on the allele frequencies. Genetic differentiation and hierarchical partition of genetic diversity were evaluated by Fst and Nm. The genetic differentiation coefficient among the populations(Fst ) was 0. 0466，which showed weak genetic variation present among populations. Gene flow(Nm)between primers varied from 1.0390 to 21 .7795 and the mean Nm was 5.1092. The UPGMA cluster figure that was constructed based on the genetic distance matrix showed that the BC and BD populations were clustered together firstly，and then be clustered with AD population. The results of the study showed the genetic diversity of the three 0. sima populations was high，and the genetic variation among populations was weak. Long-term monitoring and more analysis will be necessary to assess the impact by Penshui hydropower station on population genetic structure and diversity in 0. sima. |
| 熊美华,邵科,徐念,朱滨,史方.2013.乌江彭水水电站截流前后白甲鱼群体遗传结构初步研究[J].水生态学杂志,34(1):69-75. |
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