| “全鱼”Mx基因重组构建与转导分析 |
| Construction and Transference of "all-fish" Mx Gene Recombinant |
| 投稿时间：2012-11-19 修订日期：2012-12-05 |
| DOI： |
| 中文关键词:基因重组 “全鱼”Mx基因 PCR Southern杂交 |
| 英文关键词:Gene recombinant “All-fish” Mx gene PCR Southern blot |
| 基金项目:国家“863计划”项目（2011AA100404）；农业部大宗淡水鱼类产业技术体系常德综合试验站资助项目（CARS46-42） |
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| 中文摘要: |
| 通过分子重组将鲫Mx1基因的编码序列克隆到鲤β肌动蛋白基因启动子下游,构建成能在鱼体内表达的转"全鱼"Mx基因的重组分子。采用显微注射方法,将含有鲤β肌动蛋白基因启动子和鲫Mx1基因序列的重组片段转入草鱼受精卵中,经人工孵化和养殖获得转全鱼Mx基因草鱼。经PCR和Southern杂交检测分析,证实重组分子成功整合到2月龄转基因草鱼基因组中,为草鱼抗病育种研究奠定了基础。 |
| 英文摘要: |
| The coding sequence of Mxl gene of Carassius auratus was cloned under the control of carpp-actin gene promoter by molecular restructuring，then an all-fish Mx gene recombinant was constructed. The recombinant gene was transferred into fertilized zygotes of grass carps by micro-injection. A group of all-fish transgenic grass carps were obtained. Polymerase chain reaction (PCR) technology and Southern blot were carried out on treated grass carps fry(two months).It verified that all-fish Mx gene had integrated into the genome of grass carps. It setted the stage for disease-resisted breeding for grass carps.
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| 周伟,肖调义,苏建明,刘巧林,刘 敏,姚一彬,许宝红.2013.“全鱼”Mx基因重组构建与转导分析[J].水生态学杂志,34(1):63-68. |
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