| 异育银鲫致病性嗜水气单胞菌的分离鉴定与药敏特性研究 |
| Isolation , Identification and Antibiotic Susceptibility Test of Pathogenic Bacteria Strain from Hybridized Prussian Carp |
| 投稿时间：2012-10-08 修订日期：2012-10-19 |
| DOI： |
| 中文关键词:异育银鲫 嗜水气单胞菌 分离鉴定 药敏特性 |
| 英文关键词:Carrassius aurutus gibelio Aeromonas hydrophila isolation and identification antibiotic susceptibility |
| 基金项目:现代农业产业技术体系建设专项(CARS-46-10) |
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| 中文摘要: |
| 对江苏盐城某养殖场患出血病的异育银鲫(Carassius auratus gibelio)进行细菌分离鉴定和药敏试验,为该病的防控提供科学依据。从病死及濒死异育银鲫的肝脏、脾、肾中分离病原菌,经过形态观察、生理生化试验、16S rRNA序列分析,最终进行细菌鉴定,采用纸片扩散法进行药敏实验,通过人工回归感染试验确定分离菌株的致病性。分离的2株细菌(编号为WJ11-3和WJ11-4)均为嗜水气单胞菌(Aeromonas hydrophila),均能导致异育银鲫死亡,并出现与自然条件下基本一致的症状。药物敏感性试验结果显示,嗜水气单胞菌分离株WJ11-3对苯唑西林、林克霉素、万古霉素显示耐药性,对恩诺沙星等28种药物高度敏感;WJ11-4对苯唑西林、林克霉素、新生霉素显示耐药性,对氟苯尼考等28种药物高度敏感。本次异育银鲫的出血病由嗜水气单胞菌感染引起,可选用恩诺沙星、阿洛西林、氧氟沙星、苯唑西林、林克霉素、新生霉素等多种药物进行防治。 |
| 英文摘要: |
| The bacteria were isolated and identified from hemorrhage diseased Carrassius aurutus gibelio and drug sensitive tests were performed in order to provide a scientific basis for prevention and control of the hemorrhagic disease. Two kinds of dominant bacterial strains designated as WJll-3 and WJll—4 were isolated from the liver, spleen and kidney of the sick C. aurutus gibelio. The pathogens were identified by morphological observations，bio-chemical tests and 16S rDNA sequence analysis. The susceptibility test was done by disc diffusion method. The pathogens were identified as Aeromona hydrophila . They could lead to the death of C. aurutus gibelio，and could reproduce the diseased symptoms as occurred in wild. The antibiotic sensitivity analysis showed that WJ11-3 was resistant to oxacillin，link neomycin and vancomycin，and highly sensitive to 28 kinds of drugs，which included enrofloxacin; WJ11 4 was resistant to oxacillin，link neomycin and novobiocin，and highly sensitive to 28 kinds of drugs，which included florfenicol. Results obtained in this study indicated that many antibiotics(like enrofloxacin，azlocillin，ofloxacin，oxacillin， link neomycin and novobiocin)could be used to control and prevent hemorrhage diseased in C. aurutus gibelio，which caused by A. hydrophila. |
| 刘亚楠,梁利国,顾伟,谢骏.2012.异育银鲫致病性嗜水气单胞菌的分离鉴定与药敏特性研究[J].水生态学杂志,33(5):108-113. |
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