一株水产芽孢杆菌的筛选鉴定及其净水效果研究
Screening, Identification and Water Purification Effect of Strain Bacillus for agriculture
投稿时间:2012-09-28  修订日期:2012-11-15
DOI:
中文关键词:芽孢杆菌  筛选  鉴定  水质净化
英文关键词:Bacillus  Screening  identification  Water purification
基金项目:中国农业大学(烟台)校内科研项目(YT201105)
作者单位E-mail
李永芹* 中国农业大学烟台研究院山东 烟台 264670 lyongqin@163.com 
许乐乐 中国农业大学烟台研究院山东 烟台 264670  
陈克卫 中国农业大学烟台研究院山东 烟台 264670  
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中文摘要:
      从刺参(Stichopus japonicus)养殖池塘底泥中分离芽孢杆菌,筛选后获得1株优势目的菌株YT-2;根据形态学特征和生理生化特性结果,将其鉴定为枯草芽孢杆菌(Bacillus subtilis)。安全性试验证实,枯草芽孢杆菌YT-2对孔雀鱼(Poecilia reticulata)和南美白对虾(Penaeus vannamei)无毒性;水质净化试验结果显示,10d后菌株YT-2对养殖水体中氨氮和亚硝酸盐的降解率分别为81.9%和64.3%,表明该菌对净化养殖池塘水质有明显的作用,具有作为益生菌开发应用的潜力。
英文摘要:
      Bacillus strains were isolated and purified from the marine pond of sea cucumber. A dominant strain,Bacillus YT-2 was obtained through screening tests. Based on morphological features and results of physiological and biochemical characteristics,YT-2 was identified as Bacillus subtilis. The results of safety tests showed that the toxicity of the strain YT-2 was actually nontoxic on guppies and shrimp. And the water purification experiment indicated that the removal rates of ammonia nitrogen and nitrite by Bacillus subtilis YT-2 were 81.9 % and 64 .3 %,respectively. Therefore,Bacillus subtilis YT-2 can effectively improve the quality of water in aquacultural pond,and has potential application value as probiotics.
李永芹,许乐乐,陈克卫.2013.一株水产芽孢杆菌的筛选鉴定及其净水效果研究[J].水生态学杂志,34(1):96-100.
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