| 长石爬鮡3个地理群体遗传多样性的RAPD分析 |
| RAPD Analysis of the Genetic Diversity in Euchiloglanis longus from Lixianjiang |
| 投稿时间：2012-07-18 修订日期：2013-04-19 |
| DOI： |
| 中文关键词:长石爬鮡 遗传多样性 RAPD |
| 英文关键词:Euchiloglanis longus genetic diversity RAPD |
| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目）;云南省森林灾害预警与控制重点实验室开放基金; 西南林业大学动物学重点学科 |
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| 中文摘要: |
| 采用随机扩增多态DNA(RAPD)技术对李仙江流域景东、国庆和大寨长石爬鮡(Euchiloglanis longus)种群的遗传多样性进行了分析。从100条RAPD随机引物中筛选出20条引物,对长石爬鮡3个地理种群进行了分析;共检测到58个有效位点,其中多态位点36个,多态位点达62.07%,Nei基因多样性指数为0.2378,Shannon信息指数为0.3501。3个群体未检测到各自特有的扩增带。AMOVA分析结果显示,大多数(73.06%)的遗传变异由群体内不同个体间的遗传差异造成,26.94%的遗传变异来自于群体间。种群间的遗传分化系数(Gst)为0.2104,基因流Nm为1.8762。根据个体间遗传距离进行的PCoA分析显示,3个群体基本分开。 |
| 英文摘要: |
| Random amplified polymorphic DNA (RAPD ) technique was utilized as a means of analyzing the genetic diversity of Euchiloglanis Longus. Samples were collected from Jingdong(JD)，Guoqing (GQ)and Dazai (DZ)in Lixianjiang basin. Twenty random primers were sifted out from 100 RAPD，from which 58 useful loci were detected，with 36 were polymorphic，amounting to 62.07 %.The Nei's gene diversity and Shannon's information index of E. longus was 0. 2378 and 0. 3501 respectively. There are no unique amplified bands in the three geographical populations. AMOVA analysis indicated that 73. 06% of variance was within population and 26. 94% of variance was among population. Among the three populations，the genetic differentiation coefficient(Gst) and the gene flow(Nm)were 0. 2104 and 1.8762. Principal coordinate analysis(PCoA)，which was conducted by genetic distance among individuals，demonstrated that the three populations were almost separated. |
| 杨丽萍,周 伟.2013.长石爬鮡3个地理群体遗传多样性的RAPD分析[J].水生态学杂志,34(2):85-89. |
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