| 凡纳滨对虾不同类型血细胞的活性氧水平和酯酶活性 |
| Reactive oxygen species (ROS) production and esterase activity in different subpopulations of Litopenaeus vnanamei haemocyte |
| 投稿时间：2012-06-14 修订日期：2012-07-11 |
| DOI： |
| 中文关键词:凡纳滨对虾 血细胞 呼吸爆发 活性氧 酯酶 |
| 英文关键词:Litopenaeus vnanamei Haemocyte Respiratory burst Reactive oxygen specie Esterase |
| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目）,中国博士后科学基金 |
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| 中文摘要: |
| 应用流式细胞术（FCM）测定凡纳滨对虾（Litopenaeus vannamei）不同类型血细胞（透明细胞、小颗粒细胞和大颗粒细胞）的非诱导性和诱导性活性氧（ROS）含量以及非特异性酯酶活性，探讨不同类型血细胞的免疫功能。结果显示，透明细胞的非诱导性ROS含量最低，显著低于小颗粒细胞和大颗粒细胞（P<0.05），大颗粒细胞的非诱导性ROS含量最高。经脂多糖（LPS）刺激后，透明细胞的ROS含量没有显著变化（P>0.05），小颗粒细胞和大颗粒细胞的ROS含量显著提高（P<0.05）。大颗粒细胞的酯酶活性显著高于小颗粒细胞和透明细胞（P<0.05）。这些结果表明，三类血细胞在活性氧含量和酯酶活性的功能上存在差异，两类颗粒细胞的活性氧水平较高，大颗粒细胞的酯酶活性较强。 |
| 英文摘要: |
| To discuss the immune functions of different subpopulations (hyalinocytes, small granulocytes and large granulocytes) of Litopenaeus vannamei haemocyte, the non-induced and induced reactive oxygen species (ROS) production, and non-specific esterase activity of different haemocyte subpopulations were determined using flow cytometry (FCM). Results showed that the hyalinocytes were lest active in the non-induced ROS production, while large granulocytes were most active. After induction by LPS, no significant change of ROS production was observed in the hyalinocytes (P>0.05), and significant increases were showed in small granulocytes and large granulocytes (P<0.05). Esterase activity in large granulocytes was significantly higher than that in small granulocytes and large granulocytes (P<0.05). These results demonstrate that ROS production in small granulocytes and large granulocytes are higher than those in hyalinocytes, and esterase activity is higher in large granulocytes. |
| 冼健安,王安利,李彬,郭慧,苗玉涛,廖绍安,叶超霞.2012.凡纳滨对虾不同类型血细胞的活性氧水平和酯酶活性[J].水生态学杂志,33(4):132-137. |
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