4种渗透性抗冻剂对暗色唇鱼精子冷冻保存的影响 |
Effects of Four Penetrating Cryoprotetants on Cryopreservation of Semilabeo obscurus Sperm |
投稿时间:2012-04-11 修订日期:2012-04-11 |
DOI: |
中文关键词:渗透性抗冻剂 暗色唇鱼 精子冷冻 |
英文关键词:penetrating cryoprotectants Semilabeo obscurus sperm cryopreservation |
基金项目:云南大唐国际李仙江流域水电开发有限公司委托项目 |
|
摘要点击次数: 2202 |
全文下载次数: 2980 |
中文摘要: |
4℃冷冻保存条件下,以冷冻精子活力、寿命和细胞膜完整率为指标,检测二甲亚砜(DMSO)、甘油(Glycerol)、乙二醇(EG)和甲醇(MeOH)4种渗透性抗冻剂对暗色唇鱼(Semilabeo obscurus)鲜精以及对精子冷冻保存效果的影响。结果显示,4种渗透性抗冻剂对鲜精活力呈负作用,MeOH0.05);5%MeOH解冻后精子细胞膜完整率最高,为(85.67±1.11)%,与鲜精细胞膜完整率无显著差异(P>0.05)。研究表明,DMSO和Glycerol并不适合作为暗色唇鱼精子冷冻的抗冻剂,MeOH 和EG具有相似的保护作用,是暗色唇鱼精子冷冻保存潜在的渗透性抗冻剂。 |
英文摘要: |
The present study aimed to examine effects of four penetrating cryoprotectants(dimethyl sulfoxide,glycerol, ethylene glycol and methanol) on fresh and frozen-thawed(freezing temperature was 4℃) sperms of Semilabeo obscurus. The function of fresh and frozen-thawed sperms was evaluated by sperm motility,sperm life and sperm membrane integrity. Results obtained here showed that :four penetrating cryoprotectants presented different negative effects on fresh sperm motility and membrane integrity,methanol
|
王晓爱,杨君兴,陈小勇,潘晓赋,李再云.2012.4种渗透性抗冻剂对暗色唇鱼精子冷冻保存的影响[J].水生态学杂志,33(5):88-93. |
查看全文 查看/发表评论 下载PDF阅读器 HTML |