| 微生物制剂对养殖池塘细菌群落影响的PCR-DGGE分析 |
| Effect of Microbial Products on Bacterial Communities in Aquaculture Ponds : a PCR-DGGE Analysis |
| 投稿时间：2011-12-14 修订日期：2012-03-13 |
| DOI： |
| 中文关键词:微生物制剂 细菌群落 水体 沉积物 |
| 英文关键词:microbial product bacteria communities waters sediment |
| 基金项目:国家大宗淡水鱼类产业技术体系(农业部体系建设Nycytx-49-47);贵州省科技攻关项目“高效新型池塘养殖模式及底质改良研究”[黔科合NY字(2011)3071];贵州省渔业资源利用研发创新团队(黔科合院所创能[2011]4004) |
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| 中文摘要: |
| 为了解微生物制剂在水产养殖环境修复中的作用机制,采用聚合酶链式反应—变性梯度凝胶电泳(PCR-DGGE)方法,研究不同微生物制剂对养殖池塘上覆水和沉积物中细菌群落的影响。结果显示,施用微生物制剂前后,试验塘上覆水样品中细菌的DGGE优势条带无明显变化,细菌群落组成没有明显差异,提示微生物制剂对试验塘上覆水中的细菌群落组成影响很小。相对而言,施用过微生物制剂的池塘相对于对照池塘其细菌群落结构发生的变化较大;泼洒EM菌原粉3 000 g/hm2加芽孢杆菌原粉750 g/hm2的试验塘沉积物中细菌群落2 d内发生了较大变化,之后相对稳定,对照池塘和泼洒EM菌原粉3 000 g/hm2加光合细菌原粉1 500 g/hm2的试验塘沉积物中细菌群落组成变化很小。 |
| 英文摘要: |
| To understand the underlying mechanisms of microbial products in remediation of aquaculture environment，the bacterial communities in the overlay water and surface sediment of three aquaculture ponds，which were exposed to different microbial products or nothing as the control pond, were analyzed with polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE)method. The results showed that the DGGE profiles of water samples changed marginally after application of microbial product，indicated that the community composition of bacteria in the overlay water was less influenced by the microbial product. The bacterial communities in the treated ponds varied much more than that in the control pond during the whole experiment period. The community composition of bacteria in the surface sediment shifted significantly after application of effective microorganisms(3000g/hm2)and Bacillus(750 g/hm2)within two days，and then remained relatively stable，yet less variation was observed in the control pond as well as the pond receiving effective microorganisms(3000g/hm2)and photosynthetic bacteria(1 500g/hm2)during the whole experiment period. |
| 向燕,李建光,杨兴,关梅,胡世然.2012.微生物制剂对养殖池塘细菌群落影响的PCR-DGGE分析[J].水生态学杂志,33(2):100-104. |
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