| 微囊藻毒素在鲤体内富集的初步研究 |
| Primary Study on Bioaccumulation of Microcystin-LR in Cyprinus carpio |
| 投稿时间：2011-12-06 修订日期：2012-04-01 |
| DOI： |
| 中文关键词:鲤 微囊藻毒素 生物富集 腹腔注射 |
| 英文关键词:Cyprinus carpio MC一LR bioaccumulation intraperitoneal injection |
| 基金项目:河南省重点科技攻关计划(092102110077) |
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| 中文摘要: |
| 为了解微囊藻毒素MC-LR在鲤各组织器官中的生物富集作用,采用腹腔注射法将MC-LR纯品稀释液(0.215μg/g)注射到鲤体内,酶联免疫吸附法(ELISA)检测0、1、3、12、24和48h时肾脏、肝脏、肌肉、胆囊、空肠和卵巢中MC-LR含量分布和积累规律。结果显示,MC-LR在肾脏含量最高,均值为(1.007±0.120)μg/g(各器官均按干重计),其次是肝脏(0.490±0.060)μg/g、胆囊(0.355±0.011)μg/g、空肠(0.210±0.005)μg/g、卵巢(0.082±0.021)μg/g和肌肉(0.047±0.003)μg/g。鲤不同组织器官对MC-LR的富集能力存在较大差异,肾脏是MC-LR的主要靶器官,卵巢中也存在少量MC-LR富集,肌肉对MC-LR的生物富集量远低于其他组织器官。试验48h时,鲤体内各组织器官中和鱼缸水中MC-LR含量均有一定程度的下降,表明鲤体内对MC-LR有较强的解毒机制。 |
| 英文摘要: |
| To study the bioaccumulation of microcystin-LR(MC-LR) in different organs Cyprinus carpio，MC-LR contents were measured by the means of ELISA at 1，3，12，24 and 48 h after fish being injected with a dose of 0.215μg/g body weight MC一LR using intraperitoneal injection. The results showed that MC一LR mean content in kidney was(1 .007±0. 120) μg/g(dry weight )，followed by liver(0. 490 ±0. 060 ) μg/g，gallbladder(0.355±0. 011) μg/g，intestine(0. 210 1±0. 005) μg/g， ovarian(0.082±0.021) μg/g and muscle(0. 047±0. 003) μg/g. The bioaccumulation of MC一LR has large differences in C. carpio different organs，with the kidney being the main target organs，while the concentration of microcystin-LR in the muscle and ovarian was relatively low. At 48 h，microcystin-LR content in the analyzed tissues/organs and aquaria water was significantly decreased，which indicated that C. carpio body has a strong detoxifying mechanism for degrading MC-LR. |
| 雷庆铎,申 振,赵艳霞.2012.微囊藻毒素在鲤体内富集的初步研究[J].水生态学杂志,33(3):133-137. |
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