| 镉对凡纳滨对虾离体血细胞的毒性影响 |
| Toxic Effects of Cadmium on Haemocytes of Litopenaeus vannamei in Vitro |
| 投稿时间：2011-09-23 修订日期：2011-09-23 |
| DOI： |
| 中文关键词:镉 凡纳滨对虾 血细胞 流式细胞术 |
| 英文关键词:cadmium Litopenaeus vannamei haemocyte flow cytometry |
| 基金项目: 国家自然科学基金资助项目(30671628);国家科技支撑计划项目(2007BAD29B04;2007BAD29B06);广东省自然科学基金项目(S2011020003256);广东省重大科技专项(2009A060800019;2009A020101006);广东省教育部产学研结合资助项目(2006D90204005和2011B090500009);广州市科技支撑计划项目(2009Z1-E681) |
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| 中文摘要: |
| 对凡纳滨对虾(Litopenaeus vannamei)血细胞进行离体镉离子(Cd2+)应激试验。Cd2+浓度为10-3～10-9M,在应激6h后取样,利用流式细胞术(FCM)检测血细胞的活性和活性氧(ROS)含量。结果显示,当Cd2+浓度为10-5M时,血细胞的ROS含量显著升高;当Cd2+浓度为10-3和10-4M时,血细胞的死亡率和ROS含量均显著上升;表明Cd2+的细胞毒性作用与其浓度相关,Cd2+浓度达到10-5M时即可诱导血细胞ROS的产生,但当Cd2+浓度达到10-4M及以上时,被诱导产生的ROS对细胞造成氧化伤害,从而导致细胞死亡。 |
| 英文摘要: |
| Stress test of cadmium ion on haemocytes of Litopenaeus vannamei in vitro was conducted. Exposed to concentration of 10一9一10一3MCd2+ in 6 h in vitro，haemocyte viability and reactive oxygen species(ROS) of L.vannamei haemocyte samples were determined through flow cytometry(FCM).The results showed that ROS contents in haemocytes surged significantly under the concentration of 10一5 M cadmium; Under the concentration be-ween 10一4 and 10一3 M cadmium， mortality and ROS contents of haemocytes ascended emphatically respectively. These results showed the cytotoxicity of Cd2+ on L. vannamei was in relevance to the concentration of Cd2+. ROS of haemocytes would be induced with Cd2+ concentration reaching 10一5M，but the induced ROS would cause oxidative damage to haemocytes when Cd2+ concentration reaching 10一4 M or above，thus contributing to haemocyte death. |
| 冼健安,王安利,苗玉涛,李彬,潘训彬.2012.镉对凡纳滨对虾离体血细胞的毒性影响[J].水生态学杂志,33(1):112-115. |
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