| 稀有鮈鲫麻醉方法的研究 |
| Anesthetic Method of Gobiocypris rarus |
| 投稿时间：2010-11-11 修订日期：2010-11-11 |
| DOI： |
| 中文关键词:稀有鮈鲫 麻醉 苯佐卡因 MS-222 |
| 英文关键词:Gobiocypris rarus, anesthesia, benzocaine, MS-222 |
| 基金项目:国家自然科学基金项目（30670292）、湖北省科技基础平台专项项目及中国科学院战略生物资源技术支撑体系专项运行经费 |
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| 中文摘要: |
| 在室养条件下观察了MS-222、苯佐卡因和乌拉坦对稀有鮈鲫(Gobiocypris rarus)的麻醉效果。结果表明,乌拉坦不宜作为稀有鮈鲫的麻醉剂,而一定浓度的MS-222或苯佐卡因可导致稀有鮈鲫的快速麻醉;不同麻醉剂浓度、不同的作用时间下,稀有鮈鲫在行为上呈现不同的反应,据此可分为轻度镇静、深度镇静、轻度麻醉、中度麻醉、深度麻醉和髓质麻醉阶段;根据反应时间、恢复时间、维持时间和存活率等,建议使用60mg/L的苯佐卡因或100～110mg/L的MS-222作为深度麻醉剂量。 |
| 英文摘要: |
| As a new experimental fish，Gobiocypris rarus has been wildly used in scientific studies in China since1990. In this study，narcosis effects of MS-222，Urethan and Benzocaine on Gohiocypris rarus were observed to provide anaesthetic method for the laboratory animal. The results showed that MS-222 and Benzocaine were effective to rapidly anaesthetize for Gohiocypris rarus，whereas Urethan was disable. Sequential effects on behavior were observed when the fish exposed to different concentrations of anesthetics at different times. Based on their behaviors，low-grade sedation，deep sedation，low-grade anesthesia，medium anesthesia，deep anesthesia and medullary anesthesia stages were determined. Considering the effect time，recovery time，maintain time and survival rate，60 mg/L of Benzocaine and 100-110 mg/L of MS-222 were suggested as the suitable dosage for deep anesthesia. |
| 乔晔,胡睿,王剑伟.2011.稀有鮈鲫麻醉方法的研究[J].水生态学杂志,32(4):74-78. |
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