| 南四湖泥鳅 mtDNA 控制区遗传多样性分析 |
| Genetic Diversity Analysis of Mitochondrial D-loop Region of Misgurnus anguillicaudatus in Nansi Lake |
| 投稿时间：2010-06-11 修订日期：2010-10-20 |
| DOI： |
| 中文关键词:南四湖 泥鳅 mtDNA控制区 遗传多样性 |
| 英文关键词:Nansi Lake Misgurnus anguillicaudatus mitochondrial D-loop genetic diversity |
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| 中文摘要: |
| 利用ＰＣＲ测序技术，对采自南四湖和淮河的１２个泥鳅（Ｍｉｓｇｕｒｎｕｓａｎｇｕｉｌｌｉｃａｕｄａｔｕｓ）个体的ｍｔＤＮＡ控制区３＇端４１６ｂｐ序列进行了测序，共发现８６个多态性核苷酸变异位点，多态位点比例为０．２０６；这些核苷酸的变异位点中，５５个为插入缺失，３１个为碱基替代，变异位点的分布主要集中在６～３６、１１０～１５８、３６６～３８４核苷酸区段中，其余核苷酸区域相对保守。南阳湖、微山湖与淮河３个泥鳅种群间的遗传距离在０．０７１～０．０８０，表现出高遗传变异率。 |
| 英文摘要: |
| The genetic differences of three Misgurnus auguillicaudatus populations from Shandong Nansi Lake and Huaihe River in China were investigated by PCR coupled with sequencing. Sequence comparing showed there were 86 variation loci in the 3’ end 0f 416 bp D-loop region from 12 samples，among which 55 were from insert and deletion and 31 from base substitutions. The proportion of polymorphic loci was 0. 206. Those mutation bases are distributed within nucleotide loci of 6-36 and 110-158 as well 366-384 intensively. Other bases are relatively conservative. The net distances varied from 0. 071 to 0. 080 between groups，which exhibited high mutation rates in loach samples from the above water areas.
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| 李殿香,赵梦雨,王金星.2010.南四湖泥鳅 mtDNA 控制区遗传多样性分析[J].水生态学杂志,31(6):74-78. |
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