| 邻苯二甲酸丁基苄酯（BBP）对斑马鱼的毒性效应研究 |
| Toxic Effects of Butyl benzyl phthalate on Danio rerio |
| 投稿时间：2010-04-08 修订日期：2010-05-18 |
| DOI： |
| 中文关键词:邻苯二甲酸丁基苄酯 斑马鱼 超氧化物歧化酶 乙酰胆碱酯酶 过氧化氢酶 |
| 英文关键词:Butyl benzyl phthalate(BBP) Danio rerio SOD AChE CAT |
| 基金项目:国家自然科学基金项目(Nos.30771429.20672041) |
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| 中文摘要: |
| 通过急性毒性试验，测得邻苯二甲酸丁基苄酯（ＢＢＰ）对斑马鱼（Ｄａｎｉｏｒｅｒｉｏ）９６ｈ半致死浓度（ＬＣ５０）为３.９８ｍｇ／Ｌ；在此基础上，设置４个浓度梯度即
０.０００、０.３３２、０.６６５、１.３３０ｍｇ／Ｌ，研究了在不同的时间（７、１４、
２１、２８ｄ）暴露下，ＢＢＰ对斑马鱼超氧化物歧化酶（ＳＯＤ）、乙酰胆碱酯酶
（ＡＣｈＥ）、过氧化氢酶（ＣＡＴ）活性的影响。结果表明，随着时间的延长，ＳＯＤ活性呈现先上升、后下降的趋势，在１４ｄ时，各处理组ＳＯＤ活性达到最高水平；实验期间，各处理组ＡＣｈＥ活性被显著抑制，整体呈现下降趋势；ＣＡＴ活性在７ｄ时，除０．６６５ｍｇ／Ｌ浓度组有极显著抑制作用（Ｐ＜０．０１）外，其余各处理组ＣＡＴ活性均与对照接近，无显著差异；１４ｄ开始，除了０．３３２ｍｇ／Ｌ浓度组高于对照组，其他组与对照组相比均呈现先下降、后上升的趋势。ＢＢＰ对斑马鱼ＳＯＤ、ＡＣｈＥ、ＣＡＴ活性产生明显影响，可作为水体污染的分子生物标记物，为制订ＢＢＰ水质标准及对其进行生态风险评价提供科学依据。 |
| 英文摘要: |
| The effect of butyl benzyl phthalate，an important class of environmental endocrine disruptors，on SOD、AChE , CAT activities of Danio rerio was studied in this paper. Zebrafish were exposed to different BBP concentrations(0.000 ,0.332 ,0.665,1.330 mg/L). The activities were determined in the fish collected at 7 d，14 d ,21 d and 28 d post-exposure. It showed that SOD activity increased firstly and then declined with time extension，and was activated to the highest levels(13.51，14. 72，11.81，10. 19 U/mg) at 14d for all the treatments. From 0 d to 28 d，AChE activity was significantly inhibited. CAT activity，in 7d，except 0. 665 mg/L group，was inhibited significantly(P<0.01)，the rest groups were of no significant difference compared with the control. From 14 d，except 0. 332 mg/L group，which was always higher than the control，it showed a downward trend after the first rise for other groups. These biochemical indicators were sensitive for phthalate exposure，therefore，they can be used as potential molecular biomarkers for ecosystem risk assessment and BBP water quality criteria setting.
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| 张灿,杨 雪,何 桢,牟 文,周巧巧,胡晓静,易千慧,熊丽,刘德立.2010.邻苯二甲酸丁基苄酯（BBP）对斑马鱼的毒性效应研究[J].水生态学杂志,31(6):105-109. |
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