| 10种方蟹线粒体12S rRNA基因的序列特征和系统发育关系 |
| Characterization of Mitochondrial 12S rRNA Gene Partial Sequences and Molecular Phylogeny of Ten Species from Grapsoid Crabs |
| 投稿时间：2010-03-24 修订日期：2010-08-18 |
| DOI： |
| 中文关键词:方蟹 12S rRNA 序列 系统发生 |
| 英文关键词:Grapsoid crabs 12S rRNA Sequence Phylogeny |
| 基金项目:重庆文理学院引进人才专项(No.200803) |
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| 中文摘要: |
| 对中国沿海１０种方蟹线粒体１２ＳｒＲＮＡ基因部分片段进行了序列测定，其长度为５６５～５７６ｂｐ。它们的核苷酸序列Ａ、Ｔ、Ｇ、Ｃ的含量相似，Ａ＋Ｔ的含量（７１.９％ ～
８０.１％）明显高于Ｇ＋Ｃ的含量；１０种方蟹的１２ＳｒＲＮＡ基因序列比对获得５９２ｂｐ的同源序列（含插入／缺失位点），共检测到２３２个变异位点，其中１４１个为简约信息位
点。４种厚蟹（侧足厚蟹、天津厚蟹、日本仿厚蟹和伍氏仿厚蟹）与２种近方蟹的遗传距离（０.１０５～０.１６６）均显著小于与其它方蟹之间的遗传距离（０.２２２～０．３1５），甚至明显小于与４种厚蟹原本属于同一相手蟹科的２种相手蟹之间的遗传距离（０．２２２～０.２５２）；而基于１２ＳｒＲＮＡ基因片段序列采用ＮＪ法构建的系统进化树拓扑结构也显示，原本属于相手蟹科的４种厚蟹没有与同属该科的２种相手蟹聚为１支，而是与属于弓蟹科的２种近方蟹聚为１大支，且获得了高达１００％的支持率。结果表明，４种厚蟹与２种近方蟹的亲缘关系相对较近，而与２种相手蟹等其它方蟹的亲缘关系则相对较远，因此支持将４种厚蟹从相手蟹科移到弓蟹科；此外，属于相手蟹科的２种相手蟹聚为１支，属于方蟹科的白纹方蟹和属于斜纹蟹科的瘤突斜纹蟹又各自成为１支；表明１２ＳｒＲＮＡ基因的分子数据支持其形态学分类结果的正确性，提示上述４科蟹类可能分别为单系。 |
| 英文摘要: |
| The partial sequences of mitochondrial 12S rRNA gene of ten species crabs of Grapsoidea from the coast of China were determined and subjected to phylogenetic analysis. The lengths of sequences were from 565 to 576 bp. The A，T，G and C contents of them were similar，and the AT contents(71.9%-80. 1%)were higher than GC contents. Furthermore，592 by homologous segments of them were analyzed. The results showed that there were 232 variable sites and 141 parsimony-information sites in the nucleotides of the data. The genetic distances between four species of Helice Latimera，H. tientsinensis，Helicana japonica and H. wuana and two species of Hemigrapsus sanguineus and H. penicillatus were from 0. 105 to 0. 166 which were very much smaller than that(0.222-0. 252)between the four species of Helice and Helicana and two species of Chiromantes haematocheir and Parasesarma plicatum. However, the four species of Helice and Helicana and the two species of C. haematocheir and P.plicatum used to be believed to belong to family of Sesarmidae. Topological structure of the molecular phylogenetic tree constructed by 592 by homologous segments with Neighbor一Joining method showed that the four species of Helice and Helicana eventually were clustered into a distinct Glade(100% confidence level) with the two species of H. sanguineus and H. penicillatus. But，the two species of Hemigrapsus belonged to family of Varunidae. Therefore，the results supported that the four species of Helice and Helicana were transferred from Sesarmidae to Varunidae. In addition，the two species of C. haematocheir and P. plicatum that belong to family of Sesarmidae were clustered into a distinct Glade，and two other distinct Glades were formed by Grapsus albolineatus and Plagusia squamosa that belong to family of Grapsidae and Plagusiidae respectively.The results revealed that Grapsidae，Sesarmidae，Varunidae and Plagusiidae should be monophyletic respectively.
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| 徐敬明.2010.10种方蟹线粒体12S rRNA基因的序列特征和系统发育关系[J].水生态学杂志,31(6):69-73. |
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