| 卵形鲳!线粒体１６ＳｒＲＮＡ基因全长序列的克隆与分析 |
| Cloning and Sequence Analysis of Trachinotus ovatus Mitochondrial 16S rRNA Gene |
| 投稿时间：2010-02-09 修订日期：2010-05-22 |
| DOI： |
| 中文关键词:卵形鲳! 线粒体 １６ＳｒＲＮＡ基因 克隆 |
| 英文关键词:Trachinotus ovatus Mitochondrial 16S rRNA gene Cloning |
| 基金项目:国家科技支撑计划农业领域课题(编号2007BAD29B03) |
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| 中文摘要: |
| 根据近源物种线粒体序列的同源比对，在１６ＳｒＲＮＡ基因上、下游保守区域各设计１对通用引物。ＰＣＲ扩增获得特异的ＤＮＡ片段，经克隆、测序和比对证实，该片段包含了卵形鲳!（ＴｒａｃｈｉｎｏｔｕｓｏｖａｔｕｓＬｉｎｎａｅｕｓ）线粒体１６ＳｒＲＮＡ全长序列１７２５ｂｐ。对５个个体分别测序比对，发现卵形鲳!１６ＳｒＲＮＡ基因在钦州湾种群个体间存在至少７个变异位点，使５个个体分别具有５种不同的单倍型。将卵形鲳!与!科其它种的１６ＳｒＲＮＡ序列进行比对，构建鲳!科的系统进化树，支持!科下设４个亚科（!亚科、餷亚科、鲳!亚科、?!亚科）的分类系统。研究表明，１６ＳｒＲＮＡ基因既可用于卵形鲳!种群遗传多样性分析，又适用于!科鱼类的系统进化分析。 |
| 英文摘要: |
| Based on multiple alignment of mitochondrial sequence from neighbored spices，a pair of conserved primers was designed on the flanking region of 16S rRNA gene. The PCR amplification product was cloned to PGEM-Teasy vector and sequenced. Blast on NCBI confirmed that the sequence contain whole 1 725 bp of 16S rRNA gene. Alignment of sequence from 5 fishes of the same population revealed 7 variation sites，which form 5 different genotypes. Based on the multiple alignment of 16S rRNA sequence of Trachinotus ovatus and other Carangidae species，a phylogenetic tree was constructed which supporting the idea that Carangidae could be divided into four subfamilies: Caranginae，Seriolinae，Trachinotinae and Chorineminae. So，16S rRNA gene is a useful tool which can be used not only in genetic polymorphism analysis of Trachinotus ovatus，but also in phylogenetic analysis of Caranginae species.
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| 彭金霞,彭敏,陈秀荔,蒋伟明,杨春玲,李咏梅.2010.卵形鲳!线粒体１６ＳｒＲＮＡ基因全长序列的克隆与分析[J].水生态学杂志,31(4):81-85. |
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