| 12种中药有效成分对3株水产致病菌体外抑菌试验 |
| Antibacterial Experiment of 12 Chinese herbs active ingredient on 3 bacteria from the Aquatic Products in Vitro |
| 投稿时间：2010-01-21 修订日期：2010-04-13 |
| DOI： |
| 中文关键词:中药有效成分 温和气单胞菌 嗜水气单胞菌 变形杆菌 抑菌圈 最小抑菌浓度；最小杀菌浓度 |
| 英文关键词:Chinese herbs active ingredient Aeromonas sobria Aeromonas hydrophila Proteus vulgaris Inhibitory circle MIC MBC |
| 基金项目:科技部科技人员服务企业行动项目（2009GJF10007） |
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| 中文摘要: |
| 选择１２种有抑菌作用的中药有效成分，采用平板挖洞法对３种水产常见致病菌做药物敏感试验，然后采用二倍稀释法测定各中药有效成分对３种致病菌的最小抑菌浓度（ＭＩＣ）和最小杀菌浓度（ＭＢＣ）。结果表明：１２种中药有效成分对３株细菌都有不同程度的抑制作用，其中没食子酸、槲皮素、黄岑苷、大黄酸、盐酸小檗碱、大黄素甲醚对温和气单胞菌抑制效果最好，没食子酸、槲皮素对温和气单胞菌ＭＩＣ、ＭＢＣ最小，ＭＩＣ为０．０３１２５ｍｇ／ｍＬ，
ＭＢＣ为０.０６２５ｍｇ／ｍＬ；大黄素甲醚、没食子酸、甘草酸、槲皮素对嗜水气单胞菌抑制效果最好，大黄素甲醚、没食子酸、甘草酸对嗜水气单胞菌ＭＩＣ、ＭＢＣ最小，ＭＩＣ为０.０３１２５ｍｇ／ｍＬ，ＭＢＣ为０.０６２５ｍｇ／ｍＬ；大黄素甲醚、甘草酸、没食子酸、盐酸小檗碱对变形杆菌抑制效果为最好，没食子酸，大黄素甲醚、甘草酸对变形杆菌
ＭIＣ、ＭＢＣ均最小，ＭＩＣ为０.０３１２５ｍｇ／ｍＬ，ＭＢＣ为０.０６２５ｍｇ／mＬ。 |
| 英文摘要: |
| Sensitive test of these bacteria were carried out by disk diffusion method. Then double dilution method was used for MIC and MBC test. The results showed that the compositions of 12 Chinese herbs active ingredient can inhibit the growth of 3 bacteria in different levels，garlic acid，quercetin，baicalin，chrysophanic，berberine acid and rheochrysidin had the best effects on Aeromonas sobria，garlic acid and quercetin had the smallest MIC，MBC，MIC 0. 03125 mg/mL，MBC 0.0625 mg/mL; Rheochrysidin，garlic acid，glycyrrhizi cacid and quercetin had the best effects on Aeromonas hydrophila, rheochrysidin，garlic acid，glycyrrhizic acid had the smallest MIC，MBC，MIC0.03125 mg/mL，MBC 0. 0625 mg/mL; Physcion，Rheochrysidin，Glycyrrhizic acid，garlic acid and berberine acid had the best effects on Proteus vulgaris，rheochrysidin，Glycyrrhizic acid had the smallest MIC，MBC，MIC 0.03125 mg/mL，MBC 0.0625 mg/mL.
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| 聂鲡蓉,郑曙明,仇登高.2010.12种中药有效成分对3株水产致病菌体外抑菌试验[J].水生态学杂志,31(6):141-144. |
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