| 大口黑鲈内含子1上SNP G208A的CRS-PCR检测方法 |
| Establishment of CRS-PCR Method of Genotyping SNP G208A in the Intron 1 of the Largemouth bass (Micropterus salmoides) |
| 投稿时间：2009-10-23 |
| DOI： |
| 中文关键词:创造限制酶切位点PCR法 大口黑鲈 单核苷酸多态性 错配引物设计 |
| 英文关键词:CRS-PCR Micropterus salmoides Single nucleotide polymorphism Mismatched primer |
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| 摘要点击次数: 2077 |
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| 中文摘要: |
| 创造限制酶切位点PCR法是应用引物错配技术结合单核苷酸多态性（SNP）而配合成一个酶切位点，使SNP可用于PCR—RFLP分析的一种方法。应用CRS—PCR技术检测了大口黑鲈（Micropterus salmoides）IGF—I基因内含子1上SNPG208A的多态性，并详述了CRS—PCR错配引物的设计方法，CRS—PCR引物设计和应用的注意事项，以促进CRS—PCR单核苷酸多态检测方法在水产动物研究领域的应用。 |
| 英文摘要: |
| Created restriction site PCR （CRS-PCR） uses mismatches in one of the two PCR primers flanking the single nucleotide polymorphism to create or remove a restriction endonuclease recognition site in order to identify SNP genotypes. In this study, the genotype of SNP G208A in the intron 1 of the largemouth bass was identified with method of CRS-PCR. In addition, it was described a web-based program that facilitates the design of mismatched PCR primers to create or remove a restriction endonuclease recognition site and considerations for CRS-PCR primer design. The web-based program advanced the application of CRS-PCR in aquatic molecular breeding. |
| 李小慧,白俊杰,胡隐昌,叶星.2009.大口黑鲈内含子1上SNP G208A的CRS-PCR检测方法[J].水生态学杂志,30(5):144-148. |
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