| 光照强度对半滑舌鳎血液指标的影响 |
| The Effects of Light Intensities on Blood Parameters in Half-smooth Tongue-sole (Cynoglossus semilaevis) |
| 投稿时间：2009-12-16 修订日期：2010-01-28 |
| DOI： |
| 中文关键词:光照强度 半滑舌鳎 胁迫 |
| 英文关键词:light intensity Cynoglossus semilaevis Günther stress |
| 基金项目:国家高技术研究发展计划（863计划）项目（2007AA10Z23236103） |
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| 中文摘要: |
| 试验研究了不同光照强度对半滑舌鳎（Ｃｙｎｏｇｌｏｓｓｕｓｓｅｍｉｌａｅｖｉｓ）血液指标的影响。设置６个光照强度梯度，分别为正常养殖组（光照强度１０～３０ｌｘ，光周期为６Ｌ∶１８Ｄ）、０、１５０、６００、１３００和２０００ｌｘ组，光照周期为１２Ｌ∶
１２Ｄ。在试验的第０、２４、４８、９６和１９２ｈ取血，检测血浆中总超氧化物歧化酶（ＳＯＤ）、丙二醛（ＭＤＡ）、过氧化氢酶（ＣＡＴ）、谷胱甘肽（ＧＳＨ）、白蛋白／球蛋白（Ａ／Ｇ）、溶菌酶（ＬＺＭ）、血糖（Ｇｌｕ）、甘油三酯（ＴＧ）、胆固醇（ＣＨＯ）、丙
氨酸氨基转移酶（ＡＬＴ）、天门冬氨基转移酶（ＡＳＴ）等指标。结果显示，光照强度为６００、１３００、２０００ｌｘ组ＳＯＤ、ＣＡＴ、ＧＳＨ在胁迫２４ｈ后降至最低，随后逐渐上升；ＭＤＡ含量在胁迫４８ｈ后升至最高并与０ｈ组差异显著（Ｐ＜００５），随后逐渐上升与初始值接近。光照强度为０、１５０、６００ｌｘ组ＬＺＭ活力在胁迫４８ｈ后最低并与０ｈ组差异显著（Ｐ＜００５）。０、１５０ｌｘ组Ａ／Ｇ值在试验期间与０ｈ组差异显著（Ｐ＜００５）。Ｇｌｕ、ＴＧ、ＣＨＯ浓度在试验期间有波动，但差异不显著（Ｐ＞００５）。１５０、６００、１３００、２０００ｌｘ组在胁迫２４ｈ后的ＡＬＴ活力与０ｈ组差异显著（Ｐ＜００５），并维持较高水平。１５０、６００、１３００、２０００ｌｘ组在胁迫２４ｈ后的ＡＳＴ活力与０ｈ组差异显著（Ｐ＜０．０５），１５０、６００、１３００ｌｘ组在试验期间恢复为初始水平，而２０００ｌｘ组仍维持较高水平。 |
| 英文摘要: |
| The experiment studied the effects of light intensity on blood parameters in half-smooth tongue-sole(Cynoglossus semilaevis.The light intensity were chosen as follows: normal cultured group(light intensity is 10-30 lx， 6L: 18D)，0，150，600，1 300 and 2 000 lx，the photoperiod was set to 12L: 12D cycle. Samples were taken at 0, 24 , 48 , 96 and 192 h，superoxide dismutase(SOD)，malonaldehyde(MDA)，catalase(CAT)，reduced glutathione(GSH), albumin/globulin ratio(A/G)，lysozyme (LZM)，glucose(Glu)，triglyceride(TG)，cholesterol(CHO)，alanine aminotransferase(ALT) and aspartate aminotransferase(AST ) in plasma were asrayed. The results showed that: at 600，1 300 and 2 000 lx light intensity，SOD，CAT，GSH decreased to trough at 24 h，and increased afterwards; MDA reached peak at 48 h after stress，and showed significant differences with that of 0 h ( P<0.05)，then increased and restored to initial level. When light intensity were 0，150 , 600 lx，LZM reached trough at 48 h after stress，and showed significant differences with that of 0 h ( P<0.05).The treatments of 0 and 150 lx，there was significant difference with A/G of 0 h ( P<0.05).There was fluctuation in TG，Glu and CHO，but there was no significant differences with that of 0 h respectively(P>0.05).At light intensity of 150，600，1 300 and 2 000 lx，ALT showed significant differences to that of 0 h and maintained at a higher level. At 150,600，1 300,2 000 lx，AST of 24 h showed significant differences to that of 0 h(P<0.05)and 150,600，1 300lx restored to initial level，but 2 000 lx maintained at a higher level. |
| 邢克智,孙学亮,杨树元,王庆奎,于学全,胡金城.2010.光照强度对半滑舌鳎血液指标的影响[J].水生态学杂志,31(5):86-91. |
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