| ２株污染小球藻的除菌技术 |
| Preliminary study on purification of two microbial contaminated strains of Chlorella |
| 投稿时间：2009-12-16 修订日期：2010-03-19 |
| DOI： |
| 中文关键词:小球藻、藻种纯化、抗生素 |
| 英文关键词:Chlorella, purification of strain, antibiotics |
| 基金项目:国家自然科学基金项目（50907074） |
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| 中文摘要: |
| 在考察普通小球藻（Ｃｈｌｏｒｅｌｌａｖｕｌｇａｒｉｓ）和蛋白核小球藻（Ｃｈｌｏｒｅｌｌａｐｙｒｅｎｏｉｄｏｓａ）对青霉素、硫酸链霉素和制霉菌素等３种抗生素敏感性的基础上，研究了几种对已污染藻种除菌的方法。结果表明，２株小球藻对抗生素的敏感性有明显差异；青霉素、硫酸链霉素与制霉菌素的单独使用与联合使用对藻生长的影响差异不明显；液体培养基中加入组合抗生素能够暂时抑制藻液中杂菌的生长，但并不能得到纯培养藻种；离心清洗与抗生素结合使用能够大量除掉细菌，却使霉菌旺盛生长，说明藻菌间存在复杂的相互关系；使用添加合适浓度的混合抗生素的固体平板连续分离２次最终得到了藻的纯培养物。
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| 英文摘要: |
| Purification methods of two strains of Chlorella(Chlorella vulgaris and Chlorella pyrenoidosa) contaminated with microbes was studied by investigating sensibility of the two strains to penicillin，streptomycin sulfate and nystatin. The results revealed sensibilities of the two strains of Chlorella to antibiotics were obviously different. There was no great difference of effects between combined and individual utilization of these antibiotics on growth of the two strains. Though combination of these antibiotics could inhibit microbial contaminants temporally in liquid medium，it can not give pure culture. To unite addition of antibiotics and process of centrifuging and washing could remove most bacteria efficiently，but moulds grew up bloomy. It implied that there was a complex relationship among Chlorella，contaminative bacteria and moulds in culture media. Finally，pure culture of Chlorella strains were achieved by applying spread plate method twice with combined antibiotics at proper concentration in media.
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| 王海英,裴国凤,杨 柳.2010.２株污染小球藻的除菌技术[J].水生态学杂志,31(4):146-149. |
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