| 黄河干流宁蒙段渔业资源调查及保护对策 |
| A research and protective strategy of the fishery resource in Ningxia-Neimeng(Ning-meng) river reach of Yellow River trunk stream |
| 投稿时间：2009-12-07 修订日期：2010-01-22 |
| DOI： |
| 中文关键词:异育银鲫 温和气单胞菌 １６ＳｒＲＮＡ基因 ｇｙｒＢ基因 溶血素基因 |
| 英文关键词:Yellow River trunk stream Fishery resource Protective strategy |
| 基金项目:国家水产种质资源整理整合项目资助（2007DKA30470011） |
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| 中文摘要: |
| ２００８年１０月江苏盐城大丰精养异育银鲫（Ｃａｒａｓｓｉｕｓａｕｒａｔｕｓｇｉｂｅｌｉｏ）发生严重疾病，从病鱼肝脏、血液及腹水中分离到优势生长的细菌。对分离做纯培养的４株菌（ＪＹ０８１０１６－１～ＪＹ０８１０１６－４）进行形态特征、理化特性等表型生物学性状检验；测定了菌株（ＪＹ０８１０１６－１）的１６ＳｒＲＮＡ和ｇｙｒＢ基因序列，分析了１６ＳｒＲＮＡ和ｇｙｒＢ基因序列的同源性，并构建了系统发生树。结果表明，分离菌对供试健康异育银鲫有强致病性，菌株（ＪＹ０８１０１６－１）所扩增的１６ＳｒＲＮＡ基因序列长度为１４４８ｂｐ（ＧｅｎＢａｎｋ登录号：ＧＱ２３２７５９），所扩增的ｇｙｒＢ基因序列长度为１１７７ｂｐ（ＧｅｎＢａｎｋ登录号：ＧＱ２３２７６０），其１６ＳｒＲＮＡ和ｇｙｒＢ基因序列与ＧｅｎＢａｎｋ数据库中维氏气单胞菌和维氏气单胞菌温和生物型的１６ＳｒＲＮＡ和ｇｙｒＢ基因序列相似性均在９７％以上；根据分离菌的表型及分子特征，判定分离鉴定的４株菌为温和气单胞菌（Ａｅｒｏｍｏｎａｓｓｏｂｒｉａ）。胞外酶及溶血活性检测表明，分离菌均能产生蛋白酶、卵磷脂酶、脂酶，在含７％家兔脱纤血液营养琼脂培养基上呈β型溶血；设计的特异性引物可扩增出溶血素基因。 |
| 英文摘要: |
| In order to understanding the status of fishery resource in Ningxia-Neimeng River Reach of Yellow River Trunk Stream，the food organism resource and fish resource were researched on the river reach, in October 2007，focusing on the change and composition of plankton，the character of fish fauna and the composition of fish catch .The results showed that the plankton appear lesser in species and quantity，the fish resource present the downward trend in the river reach; then the main reasons of fish resource decline were analyzed and discussed by finding out the relevant information and analyzing the results of this survey，as mean well，some reasonable suggestions were proposed for the conservation of fish germplasm resource and the sustainable use of fishery resources in the river reach.
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| 刘晓锋,李科社,高宏伟,韩 君,张建军.2010.黄河干流宁蒙段渔业资源调查及保护对策[J].水生态学杂志,31(4):135-141. |
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