| 鱼类肠道微生物总DNA的提取 |
| Genomic total DNA extraction from Carassius auratus intestinal microorganism |
| 投稿时间：2009-11-09 修订日期：2009-12-14 |
| DOI： |
| 中文关键词:鲫 肠道细菌 DNA提取 |
| 英文关键词:Carassius auratus Enterobacteria DNA extraction |
| 基金项目:湖北省教育厅重点项目（D200618007） |
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| 中文摘要: |
| 尝试提取鲫（Ｃａｒａｓｓｉｕｓａｕｒａｔｕｓ）肠道菌群总ＤＮＡ，为研究鱼类肠道菌群结构提供依据。以鲫肠道内容物为样本，用ＰＢＳ多次洗涤，离心样品，沉淀菌体。使用试剂盒法提取肠道菌群总ＤＮＡ，电泳结果显示，样品ＤＮＡ条带明亮，无降解现象，可用于后续分子生物学试验研究；通过设计的细菌通用引物，对其１６ＳｒＤＮＡ基因进行ＰＣＲ扩增，得到较清晰的图谱，条带整齐；表明采用该方法提取鱼类肠道微生物群落的ＤＮＡ较为简单、准确、可行。 |
| 英文摘要: |
| Total DNA of intestinal tract bacteria from Carassius auratus intestinal were extracted to investigate intestinal microflora. Taking the intestinal substance of fish as sample to precipitate bacteria through PBS cleaning and centrifuge. Using Bacterial DNA Kit to extraction intestinal tract bacteria total DNA，subsequently used as templates in PCR. The electrophoresis implied that the banding-patterns of different samples were bright and non-degeneration phenomenon. The 16S rDNA was amplified from these DNA samples through a set of bacteria-specific primers，obtained the clear atlas and neatly bands. It is indicated that using this method to extract intestinal tract microflora DNA of fish is simple，accurate and feasible.
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| 汪 明,刘军,陈爱敬,赵胜军,黄峰,刘立鹤.2010.鱼类肠道微生物总DNA的提取[J].水生态学杂志,31(3):118-121. |
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