| 铜暴露对鲤鱼消化器官消化酶活性的影响 |
| Effect of Cu2+ on Digestive Enzymes Activity in the digestive organs of Cyprinus carpio |
| 投稿时间：2009-10-28 修订日期：2009-12-11 |
| DOI： |
| 中文关键词:鲤 铜离子 蛋白酶 淀粉酶 脂肪酶 酶活性 |
| 英文关键词:Cyprinus carpio Cu2+ Protease Amylase Lipase Enzyme activity |
| 基金项目:河南省重点科技攻关计划（０７２１０２１３００２７）；河南省高等学校青年骨干教师资助计划（２００７）；新乡市科技攻关计划（０８Ｎ０４０）；河南省水产养殖重点学科（０８Ｎ０４０）；河南省水产养殖重点学科 |
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| 中文摘要: |
| 实验测定了外界铜离子（Ｃｕ２＋）对鲤（Ｃｙｐｒｉｎｕｓｃａｒｐｉｏ）肝胰脏和肠道中消化酶活性的影响。结果表明，在不同Ｃｕ２＋浓度条件下，蛋白酶的活性由高到低的顺序为：肝胰脏＞中肠＞后肠＞前肠；淀粉酶活性由高到低的顺序为：中肠＞前肠＞肝胰脏＞后肠；脂肪酶活性由高到低的顺序为：肝胰脏＞后肠＞前肠＞中肠；随着Ｃｕ２＋浓度的升高，４种消化器官中的３种消化酶活性均表现出先升高、后降低的趋势；０．２８ｍｇ／Ｌ和０．５６ｍｇ／Ｌ的Ｃｕ２＋对４种消化器官中的蛋白酶和淀粉酶活性均有不同程度的激活作用，而０．８４ｍｇ／Ｌ的Ｃｕ２＋则对４种消化器官中的蛋白酶和淀粉酶活性均有不同程度的抑制作用；０．２８、０．５６和０．８４ｍｇ／Ｌ的Ｃｕ２＋对４种消化器官中的脂肪酶活性均有不同程度的激活作用；鲤肠道中的蛋白酶活性对Ｃｕ２＋的浓度变化较敏感，可以考虑用作水体Ｃｕ２＋污染的环境生物学指示。
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| 英文摘要: |
| The effect of Cu2+ on digestive enzymes activity in hepatopancreas and intestine of Cyprinus carpio was studied. The results indicate that the sequence of protease activities from highest of lowest in the digestive organs was: heptoanecteas，midgut， hindgut and foregut; The sequence of amylase activities from highest to lowest in the digestive organs was : midgut，foregut，heptoanecteas and hindgut; The sequence of lipase activities from highest to lowest in the digestive organs was: heptoanecteas，hindgut，foregut and midgut; With the rise of Cu2+ concentration，the three digestive enzymes activities in four digestive organs all rised firstly and then went down; Protease and amylase activities in four digestive organs were all enhanced by Cu2+ of 0. 28 and 0. 56 mg/L，but these digestive enzymes activities were all inhibited by Cu2+ of 0.84mg/L; Lipase activities in four digestive organs were all enhanced by Cu2+ of 0.28 , 0.56 and 0. 84 mg/L; Protease activities in intestine were sensitive to the change of Cu2+ concentration，so it has the potential to be a bio-indicator for copper pollution in water environment.
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| 江红霞,孔祥会,周晓杨,郭会芸.2010.铜暴露对鲤鱼消化器官消化酶活性的影响[J].水生态学杂志,31(4):108-112. |
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