| 氟苯尼考对大黄鱼的急性毒性及体外抑菌试验 |
| Experimental Study on Inhibiting Bacteriain Vitro and Acute Toxicity of Florfenicol to Pseudosciaena crocea |
| 投稿时间：2009-09-02 修订日期：2009-09-02 |
| DOI： |
| 中文关键词:氟苯尼考 大黄鱼 体外抑菌 急性毒性 |
| 英文关键词:florfenicol Pseudosciaena crocea inhibiting bacteriain vitro acute toxicity |
| 基金项目:农业部公益性行业科研专项（２００９０３０２９）；浙江省科技厅重点攻关项目（２００５Ｃ２３０８０）；温州市科技局科技兴海项目（Ｓ２００６００３９）。 |
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| 中文摘要: |
| 采用倍比稀释法,研究氟苯尼考对哈氏弧菌、副溶血弧菌、溶藻弧菌等8种大黄鱼(Pseudosciaena crocea)致病弧菌的体外抑菌效应;同时以棋盘微量稀释法进行五倍子、黄连、乌梅与氟苯尼考的联合用药试验,并研究了该药对养殖大黄鱼的急性毒性效应。结果表明,氟苯尼考对所选弧菌的最小抑菌浓度(MIC)在0.39～0.78μg/mL,且其MBC值与MIC值相等或为对倍关系;联合用药显示,MIC值比单独使用时略有降低,FIC指数为1.00～0.75,其中以氟苯尼考和五倍子的协同作用最为显著;氟苯尼考口服给药对大黄鱼具有较高安全性,96h LD50>1000mg/kg,对大黄鱼的安全浓度SC>100mg/kg。 |
| 英文摘要: |
| The checkerboard microdilution method was applied to study the effect of florfenicol on inhibiting bacteriain in vitro isolated from diseased Pseudosiaena crocea with disk-diffusion method. It is studied that the effect of florfenicol combined with 3 Chinese herbal medicines and florfenicol with Pseudosciaena crocea. The results showed that MIC values of florfenicol against eight strains of vibrios were in the range 0. 39-0.78 μg/mL，and the minimal bactericidal concentrations( MBC)was equal or doubled to minimal inhibitory concentrations ( MIC). Compared with the single text，the MIC values of combination were slightly lower, and the fractional inhibitory concentration(FIC)index was between 1 and 0.75. The synergic action of Rhus punjabensis Stew and florfenicol were obviously observed; It was found that its LD50(96 h)for Pseudosciaena crocea was higher than 1 000 mg/kg body weight, and the safe concentration of florfenicol was over 100 mg/kg body weight.
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| 李思源,邢晨光,李 凯,吕永林,王国良.2010.氟苯尼考对大黄鱼的急性毒性及体外抑菌试验[J].水生态学杂志,31(3):132-136. |
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