| 中国东海日本鳗鲡居群遗传结构的AFLP分析 |
| Analysis of genetic diversity of China eastsea Anguilla japonica by AFLP |
| 投稿时间：2009-05-13 修订日期：2009-08-11 |
| DOI： |
| 中文关键词:日本鳗鲡 AFLP 居群 遗传结构 遗传多样性 |
| 英文关键词:Anguilla japonica, AFLP, population, genetic structure, genetic diversity |
| 基金项目:“908”专项江苏近海海洋综合调查与评价子专题资助（JS-908-01-01） |
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| 中文摘要: |
| 采用AFLP分子标记技术，以中国东海日本鳗鲡两个群体（闽江流域玻璃鳗养成的成鳗和长江口捕获的玻璃鳗）为材料研究其遗传多样性水平及群体的遗传结构。结果表明：6对选择性扩增引物共检测到363个位点其中闽江流域群体和长江口群体的多态位点数、多态位点频率、Nei’s基因多样性系数、Shannon’s信息指数分别为228和269、 62.81%和74.10%、 0.2781和0.3077、0.4092和0.4493，日本鳗鲡这两个群体均具有较丰富的遗传变异，但闽江流域群体的明显低于长江口群体。两群体的遗传相似度为0.814，遗传距离为0.2103，在用Nei’s遗传距离构建的遗传聚类图上，两群体明显分为两支，显示了群体分化现象，不同地理群到达产卵地的时间不同可能是造成这种现象的主要原因。 |
| 英文摘要: |
| Two populations of Anguilla japonica from the East China Sea，the adult eels raised from Minjiang river’s glass eel and the glass eels from the Changjiang river were used to assess the genetic diversity and the population genetic structure by AFLP. 363 discernible DNA fragments loci were detected by using 6 selective amplify primers. Polymorphic loci, polymorphic frequencies, Nei’s index of phenotypic diversity and Shannon index were 228, 62.81%, 0.2781, 0.4092, respectively, among Changjiang population, and 269, 74.10% , 0.3077, 0.4493, respectively, among Minjiang population. The results suggested that the two East Sea eel populations had abundant genetic variation, and the Minjiang river population was lower than the Changjiang river population, which indicated that high levels of genetic variation existed in the natural populations of Chinese Anguilla japonica. Genetic similarity of the two populations was 0.814 and genetic distance was 0.2103. According to Nei’s genetic distance matrix, the individuals of the two populations were clustered and the results showed that there was genetic differentiation between the two populations. Different geographic populations to reach the nesting ground at different times may be the main reason for this phenomenon. |
| 张辛,许璞,许广平,刘培庭,仲霞铭,刘旭光,徐键荣,丁亚平,徐晓风.2010.中国东海日本鳗鲡居群遗传结构的AFLP分析[J].水生态学杂志,31(1):82-86. |
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