| 草鱼FGF8蛋白的理化特性分析 |
| Physico-chemical Properties of FGF8 in Ctenopharyngodon idellus |
| 投稿时间：2009-04-26 |
| DOI： |
| 中文关键词:草鱼 FGF8蛋 理化特性 |
| 英文关键词:Ctenopharyngodon idellus Fibroblast growth factor 8 Physico-chemical properties |
| 基金项目:重庆市自然科学基金项目(CSTC,2007BB1373 |
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| 摘要点击次数: 1932 |
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| 中文摘要: |
| 运用生物信息学手段对草鱼(Ctenopharyngodon idellus)FGF8蛋白理化特性进行分析,了解其FGF8蛋白结构及氨基酸组分。结果显示,草鱼FGF8编码的氨基酸多肽链全长210个氨基酸,包含40个碱性氨基酸、16个酸性氨基酸。蛋白质的N端区域包含1个氨基酸序列为MRLIPSRLSYLFLHLFAFCYYA的信号肽,蛋白质N-末端的信号肽被认为是分泌蛋白的明显特征,提示草鱼FGF8蛋白可能是分泌蛋白。氨基酸的一级结构中,在Asn37和Asn136氨基酸位点出现N-糖基化结构,分别为Asn-Phe-Thr和Asn-Tyr-Thr。草鱼FGF8推导蛋白的分子量Mw=24.68kDa,等电点pI=10.93。其二级结构包含α-螺旋、β-转角、β-折叠、随机卷曲等结构形式。 |
| 英文摘要: |
| Physico-chemical properties of FGF8 in Ctenopharyngodon idellus were analyzed by bioinformatics. The full - length grass carp FGF8 pro-polypeptide of 210 amino acids including 40 basic amino acids and 16 acidic amino acids. The signal peptide sequence is MRLIPSRLSYLFLHLFAFCYYA, and its cleavage site is located between Ala22 and Gln23. Grass carp FGF8 protein is a secreted protein since the typical character of secreted protein is that its N-domain contains a signal peptide. There are two N-glycosylation in the sites of Asn37 and Asn136, respectively, which were Asn-Phe-Thr and Asn-Tyr-Thr. The molecular weight （Mw） of grass carp FGF8 protein is 24.68 kDa. Its isoelectnic point （pl） is 10.93. The protein contains four main secondary structures： α-helix, β- sheet, β-turn and random coil. |
| 孙翰昌.2009.草鱼FGF8蛋白的理化特性分析[J].水生态学杂志,30(6):80-84. |
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