三峡库区长鳍吻鮈种群遗传多样性的初步研究 |
Priliminary Studies on Genetic Diversity of Rhinogobio ventralis in Three Gorges Reservoir |
投稿时间:2009-02-28 |
DOI: |
中文关键词:微卫星 长鳍吻? 三峡库区 种群遗传 多态性位点 |
英文关键词:microsatellites Rhinogobio ventralis Three Gorges Reservoir polymorphic loci population genetics |
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中文摘要: |
用磁珠富集法构建了长鳍吻?(AAAG)n富集文库,从中分离并鉴定得到13个微卫星位点。对三峡库区重庆江段的40尾长鳍吻?群体进行了遗传多样性的初步研究,其中6个位点呈现多态性(1个位点为中度多态,5个位点为高度多态),等位基因数目为4~11个,观测杂合度在0.20~0.85。这些多态性位点将为长鳍吻?及其近缘种的种群遗传研究提供有力的遗传学资料。
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英文摘要: |
From a (AAAG)n enriched genomic library, we developed 13 microsatellites loci from Rhinogobio ventralis, using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. In a test on a population of 40 individuals from Three Gorges Reservoir, Six primer pairs exhibited polymorphic with 4 to 11 alleles per locus. Observed heterozygosities ranged from 0.20 to 0.85. These loci should provide sufficient level of genetic diversity to evaluate the fine - scale population structure in the population of Rhinogobio ventralis and its congeneric species. |
徐念,史方,熊美华,阙延福,杨钟,朱滨,胡菊香,常剑波.2009.三峡库区长鳍吻鮈种群遗传多样性的初步研究[J].水生态学杂志,30(2):113-116. |
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