| 流式细胞术与显微观察对罗氏沼虾血细胞的比较研究 |
| Comparison of Flow Cytometry and Light Microscopy on the Analysis of Macrobrachium rosenbergii, Haemocytes |
| 投稿时间：2008-09-27 |
| DOI： |
| 中文关键词:流式细胞术 血细胞 罗氏沼虾 |
| 英文关键词:Flow cytometry Haemocyte Macrobrachium rosenbergii |
| 基金项目: |
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| 摘要点击次数: 2001 |
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| 中文摘要: |
| 应用流式细胞术和显微观察对罗氏沼虾（Macrobrachium rosenbergii）血细胞的分类分群和各类细胞组成比例进行研究，比较2种方法的优缺点。应用显微观察，根据细胞颗粒的有无和大小以及细胞染色情况，可把罗氏沼虾血细胞分为透明细胞、小颗粒细胞和大颗粒细胞。根据其血细胞的前向角散射光（FSC）和侧向角散射光（SSC）特性的不同，也将血细胞分为3个亚群：透明细胞、小颗粒细胞和大颗粒细胞。检测各种细胞所占的比例，2种方法测得比例均为：小颗粒细胞〉大颗粒细胞〉透明细胞；2方法的结果存在显著差异（P〈0．05），但相关性极显著（P〈0．01）。利用流式细胞术可更快捷、准确，减少人为误差，显微观察可对细胞内部结构进行更深入的分析，并可进行细胞实际大小和细胞密度的测定。2种方法互补不足，可更快速、准确、全面地进行细胞基础特征的研究。 |
| 英文摘要: |
| Haemocyte classification and the percentages of haemocyte subpopulations of Macrobrachium rosenbergii were studied by flow cytometry and light microscopy. Three kinds of subpopulations, hyalinocytes, small granulocytes and large granulocytes were distinguished by light microscopy according to the distribution, size of granules and the stain of haemocytes. By flow cytometry, the haemocytes were divided into the same three distinct subpopulations according to forward light scatter（FSC） and side light scatter（ SSC ） signals from each individual. The proportion in the hemocytes ： small granulocytes 〉 large granulocytes 〉 hyalinocytes. The two methods have the significant differences in the statistics of the percentages of these three subpopulations, but the correlation between them was high significant. Flow cytometry is a powerful analytical technique of haemocytes, but the cellular inner parameters, sizes and the densities of the haemocytes can be measured only by light microscopy. Using the both methods synthetically, the haemocyte parameters can be analysed more rapidly, accurately and comprehensively. |
| 冼健安,王安利.2009.流式细胞术与显微观察对罗氏沼虾血细胞的比较研究[J].水生态学杂志,30(5):90-94. |
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