| 转基因鱼试验湖浮游生物群落DNA指纹与理化因子的关系 |
| Relationship between DNA Fingerprinting Structure of Plankton Community and Physicochemical Factors in an Artificial Lake |
| 投稿时间：2008-09-16 |
| DOI： |
| 中文关键词:ＰＣＲ－ＤＧＧＥ 理化因子 ＤＮＡ多态性 浮游生物群落 转基因鱼试验湖 |
| 英文关键词:PCR - DGGE physicochemical factors DNA polymorphism plankton community tentative lake of transgenic fish |
| 基金项目: |
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| 中文摘要: |
| 对转基因鱼试验湖７个采样点的浮游生物群落进行了ＰＣＲ－ＤＧＧＥ指纹分析，并进而探讨了ＤＮＡ指纹与理化因子的关系。结果表明：（１）ＰＣＲ－ＤＧＧＥ指纹图谱中共含１０４谱带，其中原核谱带５８条，真核谱带４６条，其多态性位点分别为８７．９％和８２．６％。（２）各站点的理化因子中，Ⅰ站的ＴＰ含量最高（０．１０ｍｇ／Ｌ），ＴＮ含量在Ⅲ站最高（０．３４ｍｇ／Ｌ），且各站点波动较大，Ⅴ站的透明度最低（６３．００ｃｍ）；ＮＯ３－Ｎ、ＮＨ４－Ｎ、ｐＨ、ＤＯ、ＣＯＤ及电导率在各站点变化不大。基于原核生物图谱的相似性聚类分析表明，浮游生物群落Ⅵ和Ⅶ、Ⅳ和Ⅴ、Ⅱ和Ⅲ相似度分别较高，Ⅰ站有别于其它各站，单独为１枝；基于真核生物图谱的聚类分析显示，Ⅰ、Ⅱ站聚为１枝，Ⅳ、Ⅴ、Ⅵ和Ⅶ依次相聚后与Ⅲ站聚为另１大枝，Ⅰ、Ⅱ站与Ⅲ站相距较近；理化因子的主成分分析结果与之基本吻合。因此，浮游生物群落ＤＮＡ多态性与环境理化因子是密切相关的，这类资料的积累将为在分子水平上建立水质预测预报体系奠定基础，为评价转基因鱼生态安全性提供依据。
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| 英文摘要: |
| To explore the relationship between community composition and physicochemical factors in an artificial lake, plankton communities from the lake for transgenic fish culture were studied by PCR - DGGE fingerprinting. The results as follows： （1） a total of 104 distinct OTUs were identified in the PCR -DGGE analysis, of which 58 were bacterial OTUs and 46 were eukaryotic OTUs, the polymorphism sites were 87.9% and 82.6% respectively; （2） the content of TP at station Ⅰ was the highest （0. 10mg/L）, while station Ⅲ consisted of the most TN （0. 34mg/L） and station Ⅴ had the lowest transparency （63.00 cm） ; the discrepancy of other measured factors were not obvious in different stations. Cluster analysis based on bacterial OTUs and the principal component analysis of physicochemical factors showed that the similarity of Ⅵ and Ⅶ, Ⅳ andⅤ, Ⅱ and Ⅲ were higher, station Ⅰ was a single cluster; cluster analysis based on eukaryotic OTUs revealed that the seven stations could be grouped into two clusters ： the cluster one contained the station Ⅰ and Ⅱ , and another cluster consists of the other stations. In conclusion, the DNA fingerprinting of plankton community was closely related to the environmental physicochemical factors, and the data of such kind accumulated will play an important role in constructing a forecast system for water quality, and provide a basis to evaluate the ecological safety of transgenic fish. |
| 李学梅,余育和,冯伟松,颜庆云,敖鸿毅.2008.转基因鱼试验湖浮游生物群落DNA指纹与理化因子的关系[J].水生态学杂志,29(1):20-24. |
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