| 液相色谱-串联质谱法测定水产饲料中喹乙醇残留量 |
| Determination of Olaquindox Residues in Aquatic Feed by High Performance Liquid Chromatography-Tandem Mass Spectrometry |
| 投稿时间：2007-10-17 |
| DOI： |
| 中文关键词:液相色谱－串联质谱 固相萃取 喹乙醇 水产饲料 |
| 英文关键词:high performance liquid chromatography-tandem mass spectrometry solid phase extraction Olaquindox aquatic feeds |
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| 中文摘要: |
| 为测定水产饲料中喹乙醇的残留量，建立了高效液相色谱－串联质谱的测定方法。水产饲料经５％甲醇溶液提取，ＨＬＢ固相萃取小柱净化后上机定量检测。采用ＺｏｒｂａｘＸＤＢＣ１８色谱柱，并以０．２％乙酸水溶液＋甲醇（６０＋４０，体积分数）为流动相，色谱分离后直接进串联质谱器。采用电喷雾正离子，多反应监测模式检测，外标法定量。在１～２０ｍｇ／Ｌ浓度下具有良好的线性关系，方法的定量限为０．０４ｍｇ／ｋｇ，添加浓度在１～２０ｍｇ／ｋｇ时的回收率为７２．２％ ～９８．５％，相对标准偏差小于７．９％。
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| 英文摘要: |
| A high performance liquid chromatography-tandem mass spectrometric （ LC - MS/MS） method was de veloped for determination of olaquindox residues in aquatic feeds. Olaquindox in feeds were extracted with 5% methanol solution and further purified with HLB solid phase extraction cartridge. Chromatographic separation was a- chieved by using Zorbax XDB C18 column with an isoeratic mobile phase consisting of 0.2% acetic acid and methanol （60 ＋ 40, V/V）. Analytical identification and quantification were performed using multiple reaction monitoring with one precursor ion and three product ions as identifiers and electrospray ionization in positive mode. It was linearly ranged from 1 to 20mg/L, the LOQ was 0.04mg/kg. the recoveries were 72.2% -98.5% , the RSD was lower than 7.9% |
| 张海琪,何中央,徐晓林,施礼科.2009.液相色谱-串联质谱法测定水产饲料中喹乙醇残留量[J].水生态学杂志,30(1):131-134. |
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