| 几种金属离子对鲢肝胰脏淀粉酶的影响 |
| Effects of Metal Ions on Activities of Amylase from Silver Carp (Hypophthalmichthys molitrix) Hepatopancreatic |
| 投稿时间：2008-04-08 |
| DOI： |
| 中文关键词:鲢 淀粉酶 酶活力 金属离子 |
| 英文关键词:Silver carp （Hypophthalmichthys molitrix） Amylase Amylase activity Metal ions |
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| 中文摘要: |
| 以鲢的肝胰脏为材料，取组织匀浆上清液作为酶液进行研究。结果表明：鲢肝胰脏淀粉酶的最适pH为6．8，最适温度为35℃，最适底物浓度为0．03％。进一步研究1、2价金属离子和重金属离子对鲢肝胰脏淀粉酶活力的影响，结果表明：1价金属离子K＋、Na＋对酶活力没有影响，2价金属离子Ba2＋、Ca2＋对酶活力具有抑制作用，重金属离子Cd2＋、Hg2＋对酶活力具有显著的抑制作用。 |
| 英文摘要: |
| The characteristics of amylase from Silver carp （Hypophthalmichthys molitrix） hepatopancreatic were studied. The supernatant of tissue homogenate was taken as the mother liquor for pilot study. The results showed that ： the optimum pH of amylase activity was 6.8, the optimal temperature was 35℃, and the optimal fit substrate concentration was 0.03 %. The effects of some metal ions on the amylase activity were also studied. It was showed that the positive univalent metal ions （K ＋ and Na＋ ） had little influence on the enzyme activity, the positive bivalent metal ions （Ba2 ＋ and Ca2 ＋ ） inhibited the enzyme activity, and heavy metal ions （Cd2 ＋ and Hg2＋ ） inhibited strongly the enzyme activity. |
| 辛碧芬,谢进金,庄巧阳.2009.几种金属离子对鲢肝胰脏淀粉酶的影响[J].水生态学杂志,30(3):91-95. |
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