环境因素对淡水浮丝藻生长及产生嗅味的影响
Effects of Environmental Factors on the Growth and 2-MIB Production of Planktothrix sp.
投稿时间:2020-08-18  修订日期:2020-10-14
中文关键词:浮丝藻  二-甲基异莰醇  温度  营养盐浓度  光照强度
英文关键词:Planktothrix sp.  2-Methylisoborneol (MIB)  temperature  nutrient concentration  illumination intensity
基金项目:国家自然科学基金项目(21766025);内蒙古自治区科技计划项目(2020年度);中国科学院“西部之光”人才培养引进计划“西部青年学者”项目;内蒙古科技大学创新基金项目-优秀青年科学基金项目(2017YQL03);内蒙古科技大学创新基金项目(2019QDL-B20);内蒙古自治区教育厅,高等学校科学研究项目(NJZY21402)
作者单位
成建国 内蒙古科技大学化学与化工学院内蒙古 包头014010生物煤化工综合利用内蒙古自治区工程研究中心内蒙古 包头014010 
马力通 内蒙古科技大学化学与化工学院内蒙古 包头014010生物煤化工综合利用内蒙古自治区工程研究中心内蒙古 包头014010 
赵文渊 内蒙古科技大学化学与化工学院内蒙古 包头014010 
刘云颖 内蒙古科技大学化学与化工学院内蒙古 包头014010生物煤化工综合利用内蒙古自治区工程研究中心内蒙古 包头014010 
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中文摘要:
      研究温度、营养盐浓度以及光照条件对淡水浮丝藻(Planktothrix sp.) 及其代谢产物2-MIB的影响,为控制丝状蓝藻生长及预警水体嗅味物质污染提供实践依据。浮丝藻由中国科学院水生生物研究所淡水藻种库分离于安徽巢湖;设置的试验温度为15、20、25、30℃,营养盐溶液体积分数为20%、30%、50%、100%,光照为1000、2000、3000、4000 lx。结果表明,温度对浮丝藻生长密度、总2-MIB生成量及胞外2-MIB分泌量影响最显著,25 ℃是浮丝藻最适宜生长温度,最高细胞密度可达6.04×105 个/mL,30 ℃是其最适宜的产2-MIB温度,总2-MIB生成量和胞外2-MIB分泌量均最高,分别为3.64×103 ng/L和2.78×103 ng/L;其次为营养盐浓度,增大BG-11营养盐浓度,浮丝藻生长密度和总2-MIB生成量会随之增加,但低浓度营养盐时胞外2-MIB分泌量所占比例较高,表明低浓度营养盐更有利于浮丝藻进行胞外2-MIB分泌;光照对浮丝藻生长及产嗅味物质影响较小,强光照可促进2-MIB胞外释放,4000 lx时其胞外2-MIB分泌量达最高。通过调节湖库蓄水量改变水温、小型饮用水供水湖库可以引入水质较好的蓄水水源降低水库富营养程度,可避免蓝藻水华和嗅味物质污染事件的发生。
英文摘要:
      Planktothrix sp. is a cyanobacteria species broadly distributed in water bodies across China and can produce massive blooms under favorable conditions. One of its secondary metabolites, 2-methylisoborneol (2-MIB), dramatically decreases the sensory quality of drinking water because of its strong, undesirable musty odor. In this study, we investigated the effects of water temperature, nutrient concentration (BG-11 media) and light intensity on the growth of Planktothrix sp., and the production and extracellular secretion of 2-MIB. The Planktothrix sp. FACHB-1372 used in this study was isolated from Chaohu Lake and cultured in medium to the logarithmic phase with a density of 3-5×106 cell/mL. Treatments included four temperatures (15, 20, 25, 30℃), four BG-11 concentrations (20%, 30%, 50%, 100%) and four illumination intensities (1000, 2000, 3000, 4000 lx). All experiments lasted 14 days, and Planktothrix sp. samples were collected every 2 days for determination of cell density and 2-MIB content. Results show that, under the experimental conditions, temperature had the most largest effect on cell growth, 2-MIB production and secretion. The optimal growth temperature of Planktothrix sp. was 25℃, yielding a cell density of 6.04×105 cell/mL. Maximum 2-MIB production (3.64×103 ng/L) and secretion (2.78×103 ng/L) occurred at 30℃. At the lowest temperature (15℃) Planktothrix sp. grew slowly and 2-MIB production was not detected. Cell growth and total 2-MIB production increased with BG-11 concentration, but secretion of 2-MIB was higher at the low BG-11 concentration, an indication that low nutrient concentration promotes secretion of 2-MIB. Light intensity had the least effect on Planktothrix sp. growth, but growth did increase with illumination intensity from 1000-3000 lx. High illumination intensity also promoted the release of 2-MIB, with secretion reaching the highest level at 4000 lx. Thus, to avoid and control Planktothrix sp. blooms and odor problems, we recommend regulating water storage in reservoirs to minimize water temperature, and transferring water of good quality to small drinking water reservoirs where eutrophication can be more effectively controlled. This study provides a practical basis for control and early warning of Planktothrix sp. blooms and the production of undesirable odors.
成建国,马力通,赵文渊,刘云颖.2022.环境因素对淡水浮丝藻生长及产生嗅味的影响[J].水生态学杂志,43(2):70-76.
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