3种细菌对小球藻生长和脱氮除磷效率的影响
Effects of Three Bacteria on the Growth and Nitrogen and Phosphorus Removal Efficiency of Chlorella pyrenoidosa
投稿时间:2019-04-14  修订日期:2021-03-22
中文关键词:藻菌共生  蛋白核小球藻  污水处理
英文关键词:microalgal-bacterial symbiosis  Chlorella pyrenoidosa  waste water treatment
基金项目:“十三五”国家水专项(2017ZX07108-001)
作者单位
肖 伟 湖北工业大学土木建筑与环境学院湖北 武汉430068 
吴红艳 湖北工业大学土木建筑与环境学院湖北 武汉430068 
毕永红 中国科学院水生生物研究所淡水生态与生物技术国家重点研究室湖北 武汉 430072 
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中文摘要:
      鉴于水资源日益紧缺且水污染问题日渐加剧的现状,藻菌共生系统作为一种节能环保的污水处理方式,有助于提高污水处理效率。通过人工模拟城市污水,选取3种污水处理中常用细菌地衣芽孢杆菌(Bacillus licheniformis)、枯草芽孢杆菌(Bacillus subtilis)和沼泽红假单胞菌(Rhodopseudomonas palustris)构建藻菌共生系统,探究了不同细菌对蛋白核小球藻(Chlorella pyrenoidosa FACHB-5)生长以及污水脱氮除磷效率的影响。结果显示,3种藻菌共生系统中,地衣芽孢杆菌为小球藻促生长菌,枯草芽孢杆菌对小球藻生长有抑制作用,沼泽红假单胞菌对小球藻生长无显著影响(P>0.05),且沼泽红假单胞菌的生长受到小球藻的显著抑制(P<0.05)。在3个共生系统中,地衣芽孢杆菌-小球藻共生系统中小球藻生物量最高,第2天水体的总氮和总磷去除率分别达到80.28%和70.94%,显著高于小球藻单独培养试验组(P<0.05)。研究表明,3种藻菌共生系统中,细菌与微藻间的关系具有特异性,应选择适宜的促生菌与微藻共培养,合适的藻菌共生系统可提高水体总氮和总磷的去除率。
英文摘要:
      The interactions of algal-bacterial consortia can improve the efficiency of waste water treatment and are characterized by energy-saving and environmental friendliness.In this study, three commonly used bacteria (Bacillus licheniformis, Bacillus subtilis and Rhodopseudomonas palustris) were selected to coculture with Chlorella pyrenoidosa (FACHB 5) to construct the algal-bacterial symbiotic system.The effects of the different bacteria on the growth of C.pyrenoidosa and the efficiency of nitrogen and phosphorus removal from waste water were investigated and compared.B.licheniformis, B.subtilis, R.palustris and C.pyrenoidosa (FACHB 5) in the exponential growth phase were mix-cultured in an artificially composed urban sewage, at initial concentrations of 1×10^7 CFU/mL for the three bacteria and2×10^6 cells/mL for the algae. Each trial was run in triplicate with acontrol group, the trials lasted7days and the culture was sampled each 24 h.B.licheniformis promoted algal growth, B.subtilis inhibited algal growth, while R.palustris had no significant effect on algal growth (P >0.05).On the contrary, C.pyrenoidosa FACHBG5 significantly inhibited the growth of R.palustris (P <0.05).The maximum algal biomass appeared in the C.pyrenoidosa-B.licheniformis symbiotic system, and the removal rates of TN and TP in this system reached79.84% and83.48%, respectively, significantly higher than in the control (algae only) (P <0.05).Our study indicated that the interactions between bacteria and microalgae for the three algal-bacterial symbiotic systems were very different, so it is important to select suitable probiotics for the algal-bacterial consortium.The symbiotic interactions in a properly composed algal-bacterial consortia can remove TN and TP from water more efficiently than microalgae alone.
肖 伟,吴红艳,毕永红.2021.3种细菌对小球藻生长和脱氮除磷效率的影响[J].水生态学杂志,42(2):124-130.
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