| DNA条形码技术在藻类分类学中的研究现状与展望 |
| Research Status and Prospects for DNA Barcode Technology in Algal Taxonomy |
| 投稿时间：2018-03-26 修订日期：2020-12-02 |
| DOI：10.15928/j.1674-3075.2020.06.002 |
| 中文关键词:藻类 DNA条形码 物种分类 应用研究 |
| 英文关键词:algae DNA barcodes species classification biodiversity studies taxonomy |
| 基金项目:中央级公益性科研院所基本科研业务专项（2016YSKY-006） |
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| 中文摘要: |
| 在对DNA条形码（DNA barcode）技术发展进程分析归纳的基础上，对已发表的各门藻类DNA条形码序列进行总结。现阶段较为常用的DNA条形码基因主要包括ITS、cox1、rbcL等基因，几种常用基因片段的各自存在优缺点，本文对各片段的主要问题进行了分析。其中原核蓝藻的ITS基因和藻胆蛋白基因可考虑作为首选DNA条形码，真核藻类主要考虑ITS和rbcL基因，很难找到像动物cox1基因可在物种鉴定中广泛通用的DNA条形码。不同基因片段间互补应用，并与传统形态学鉴定方法相结合可有效提高近缘藻类分类的准确性。综述了藻类DNA条形码研究的新进展，展望其应用前景。认为目前藻类DNA条形码的研究重点仍是加快开发新的DNA条形码片段并对其进行评价，为寻找理想的通用DNA条形码提供理论支持。 |
| 英文摘要: |
| Barcodes for the DNA sequences of algal genes have gradually come into use because they provide a quick, effective means for identifying algae in biodiversity studies. In this study, we summarized commonly used barcodes of algae genes and, based on an analysis of DNA barcode technology, identified their advantages and disadvantages. At present, the most commonly barcoded genes include ITS, cox1, rbcL. Barcodes of DNA sequences in the ITS and phycobiliprotein genes is now the preferred method of identifying prokaryotic cyanobacteria, and barcodes of the ITS and rbcL genes have been found suitable for eukaryotic algae. However, it should be noted that finding a suitable gene for DNA barcoding in animals, that can be widely used for species identification, has proven difficult. Therefore, current research on DNA barcoding needs to focus on accelerating the development of new DNA barcode fragments for algae and evaluating their utility. This will provide theoretical support in the search for ideal universal DNA barcodes. Furthermore, traditional morphological identification methods, complemented by gene fragment identification, will improve accuracy when classifying closely related algae species. |
| 毛启迪,杨苏文,金位栋,闫振广,闫玉红,焦立新,徐彬,焦巨龙.2020.DNA条形码技术在藻类分类学中的研究现状与展望[J].水生态学杂志,41(6):9-18. |
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