两种微藻对分散橙S-4RL胁迫的光合活性响应
Effect of Disperse Orange S-4RL on the Photosynthetic Activity of Two Freshwater Algae
投稿时间:2017-12-09  修订日期:2019-03-20
中文关键词:分散橙S-4RL  胁迫  小球藻  水华微囊藻  光合活性
英文关键词:Disperse orange S-4RL  stress  Chlorella vulgaris  Microcystis flos-aquae  photosynthetic activity
基金项目:福建省中青年教师教育科研项目(JAT160029);石狮市科技计划项目(2016FS21)
作者单位E-mail
李雪妍 华侨大学 化工学院福建 厦门 361021 15205032698@163.com 
李玲 华侨大学 化工学院福建 厦门 361021 liling19830826@hqu.edu.cn 
王焕双 华侨大学 化工学院福建 厦门 361021 495209510@qq.com 
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中文摘要:
      探究环境中分散橙S-4RL对水生藻类的污染胁迫,为评估合成染料对水生生态环境的风险提供参考依据。分散橙S-4RL(C.I.分散橙30)纯度≥95%,水华微囊藻(Microcystisflos-aquae)、蛋白核小球藻(Chlorella pyrenoidosa)按照OECD 201藻类生长试验规定培养。最终染毒浓度分别为0.625、1.25、2.5、5、10 mg/L,使用多脉冲调制叶绿素荧光仪每24h测定叶绿素a含量1次,其他叶绿素荧光参数每隔48 h测定1次。结果表明:(1)在试验浓度范围内,分散橙S-4RL对蛋白核小球藻的叶绿素a含量具有促进作用,其中最低浓度(0.625 mg/L)处理组在第6天达到了空白对照组的115%;而分散橙S-4RL对水华微囊藻的叶绿素a含量均呈现抑制作用。(2) 分散橙S-4RL对蛋白核小球藻和水华微囊藻光合反应过程最大光能转换效率(Fv/Fm),PSⅡ的潜在活性(Fv/F0)以及初始斜率(α)影响不大;但蛋白核小球藻和水华微囊藻的最大电子传递速率(ETRmax)和半饱和光强点(Ik)均随着分散橙S-4RL浓度增加而增加,在试验第5天,水华微囊藻的ETRmax和Ik均为空白对照组的119%。2种微藻对分散橙S-4RL的胁迫敏感性不同,但都能提高光合活性来缓解胁迫。
英文摘要:
      Dyes are increasingly selected based on resistance to light, washing and perspiration, and to meet consumer demand for color diversity. However, dyes inevitable enter the aquatic environment during production and use, resulting in risk to aquatic ecosystems. To evaluate the toxicity of Disperse Orange S-4RL (DO S-4RL) to freshwater algae, we investigated the effects of low level exposure to DO S-4RL on the photosynthetic activity of Chlorella pyrenoidosa (green alga) and Microcystis flos-aquae (Cyanophyta) using a color pulse amplitude modulation chlorophyll fluorimeter (Phyto-PAM). The study provides a reference for risk evaluation of dyes in the aquatic environment. Chlorella pyrenoidosa and Microcystis flos-aquae were cultured following OECD guidelines and algae in logarithmic phase were exposed to different concentrations of DO S-4RL (0.625, 1.25, 2.5, 5, 10 mg/L). Each treatment and a control were run in triplicate. The test duration was six days and the content of chlorophyll-a in each group was measured each 24 hours and chlorophyll fluorescence parameters were determined each 48 hours. Exposure to DO S-4RL increased the chlorophyll-a content of Chlorella pyrenoidosa, especially in the low concentration (0.625 mg?L-1) group, in which the chlorophyll-a content was 115% of that in the control group on day 6. However, DO S-4RL decreased the chlorophyll-a content of Microcystis flos-aqua. Exposure to DO S-4RL had no significant effect on the maximum photochemical quantum yield (Fv/Fm), the potential activity (Fv/F0) or the initial slope (α) of Chlorella pyrenoidosa and Microcystis flos-aquae. With increased DO S-4RL concentration, the values of ETRmax and Ik of Chlorella pyrenoidosa and Microcystis flos-aquae increased. The values of ETRmax and Ik of Microcystis flos-aquae in all treatments were 119% of those in the control group on day 5. The sensitivities of the two microalgae species to DO S-4RL were different, but both increase photosynthetic activity to relieve the stress.
李雪妍,李玲,王焕双.2019.两种微藻对分散橙S-4RL胁迫的光合活性响应[J].水生态学杂志,40(2):73-81.
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