中华绒螯蟹生态育苗池塘细菌群落分析
Microbial Community in Ecological Breeding Ponds for the Chinese Mitten Crab Eriocheir sinensis
投稿时间:2017-07-31  修订日期:2019-01-14
中文关键词:中华绒螯蟹  生态育苗  养殖水体  底泥
英文关键词:Eriocheir sinensis  ecological breeding ponds  aquiculture water  sediment
基金项目:江苏省水产三新项目(D2017-4-2);江苏省科技厅现代农业项目(BE2015369)
作者单位E-mail
徐 宇 江苏省淡水水产研究所江苏 南京 210017江苏省淡水水产种质资源保护与利用重点实验室江苏 南京 210017江苏省农业种质资源保护与利用平台江苏 南京 210014 jsgyxuyu@126.com 
邓燕飞 江苏省淡水水产研究所江苏 南京 210017江苏省淡水水产种质资源保护与利用重点实验室江苏 南京 210017江苏省农业种质资源保护与利用平台江苏 南京 210014 yf-deng@163.com 
杨志强 江苏省淡水水产研究所江苏 南京 210017江苏省淡水水产种质资源保护与利用重点实验室江苏 南京 210017 江苏省农业种质资源保护与利用平台江苏 南京 210014 1242432873@qq.com 
葛家春 江苏省淡水水产研究所江苏 南京 210017江苏省淡水水产种质资源保护与利用重点实验室江苏 南京 210017 江苏省农业种质资源保护与利用平台江苏 南京 210014 gjco9@sing.com 
杨鸢劼 江苏省淡水水产研究所江苏 南京 210017 江苏省淡水水产种质资源保护与利用重点实验室江苏 南京 210017 江苏省农业种质资源保护与利用平台江苏 南京 210014 南京淳一水产科技发展有限公司江苏 高淳 211316 yangyj025@126.com 
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中文摘要:
      为探究江苏省射阳地区沿海滩涂中华绒螯蟹(Eriocheir sinensis)生态育苗池塘细菌群落结构,分别对养殖池塘水体(S7,S8,S9)和池塘底泥(N7,N8,N9)细菌群落16S rDNA的V3、V4、V5可变区进行扩增,检测合格的文库进行高通量测序;利用Qiime(v1.7)软件,对序列阈值97%相似性水平进行聚类分析;分别以门、纲、目、科和属的分类水平对样本进行α-多样性指数分析;以Chao指数和Shannon指数评估菌种丰富度和多样性。结果表明,3个生态育苗池塘得到OTU分别为8 881、7 406、8 566个。S7微生物归属于16门、29纲、58目、97科、191属;S8为9门、18纲、38目、68科、132属;S9为17门、32纲、60目、111科、227属。N7为41门、103纲、198目、325科、572属;N8为42门、103纲、203目、318科、534属;N9为40门、99纲、196目、323科、578属。养殖水体优势菌群以变形菌门(Proteobacteria)为主,其次是拟杆菌门(Bacteroidetes);池塘底泥优势菌群以变形菌门(Proteobacteria)为主,其次为拟杆菌门(Bacteroidetes)、绿弯菌门(Chloroflexi)和浮霉菌门(Planctomycetes)。养殖池塘底泥中细菌群落的丰富度和物种多样性明显高于养殖水体,水体中细菌群落的丰富度S9> S7> S8,物种的多样性S7> S9> S8。池塘底泥中细菌群落的丰富度N7> N9> N8,物种的多样性N7> N9>N8。
英文摘要:
      Microbial community composition plays an important role in aquaculture systems, affecting the nutrition and immune response of aquaculture organisms. In this study, we investigated the microbial community structure and diversity in the water and sediment of Chinese mitten crab ecological breeding ponds in Sheyang, Jiangsu Province, aiming to provide a reference for environmental protection and healthy breeding of the crab. Water and sediment samples (labeled as S7,S8,S9 and N7,N8,N9) were collected from three breeding ponds in the aquatic seedling farm of Sheyang County. The V3–V5 hypervariable region of the 16S rRNA gene from bacteria in water and sediment samples was sequenced using high-throughput sequencing technology and expressed in operational taxonomic units (OTUs) using MOTHUR software. The resulting sequences with less than 97% sequence similarity were clustered using Qiime(v1.7)software and the richness and diversity of the microbial community were evaluated based on the Chaol index and Shannon index. Ponds 7, 8, and 9 displayed, respectively, 8881, 7406 and 8566 OTUs. The microbial community of water sample S7 included 191 genera, 97 families, 58 orders, 29 classes and 16 phyla; for S8, 132 genera, 68 families, 38 orders, 18 classes and 9 phyla; and for S9, 227genera, 111 families, 60 orders, 32 classes and 17 phyla. The microbial community of sediment sample N7 included 572 genera, 352 families, 198 orders, 103 classes and 41 phyla; for N8, 534 genera, 318 families, 203 orders, 103 classes and 42 phyla; and for N9, 578 genera, 323 families, 196 orders, 99 classes and 40 phyla. Proteobacteria was the dominant taxa detected in the water and sediment samples, followed by Bacteroidetes. The abundance and diversity of microbes in the sediment were much higher than in the water. The information provided by this study will be helpful in rearing Chinese mitten crab larvae.
徐 宇,邓燕飞,杨志强,葛家春,杨鸢劼.2019.中华绒螯蟹生态育苗池塘细菌群落分析[J].水生态学杂志,40(1):64-70.
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