溶氧胁迫对异育银鲫感染鲫造血器官坏死症的影响
Effect of Dissolved Oxygen Stress on Hematopoietic Necrosis Infection in Carassius auratus gibelio
投稿时间:2017-07-28  修订日期:2018-11-18
中文关键词:异育银鲫  溶氧胁迫  鲤疱疹病毒Ⅱ型  感染
英文关键词:Carassius auratus gibelio  dissolved oxygen stress  Cyprinid herpesvirus II  infection
基金项目:国家大宗淡水鱼类产业技术体系建设专项资金项目( CARS-46-37)
作者单位E-mail
赵彦华 江苏省淡水水产研究所江苏 南京 210017 njzhaoyanhua@163.com 
孙梦玲 江苏省淡水水产研究所江苏 南京 210017  
万金娟 江苏省淡水水产研究所江苏 南京 210017  
夏爱军 江苏省淡水水产研究所江苏 南京 210017  
薛 晖 江苏省淡水水产研究所江苏 南京 210017  
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中文摘要:
      随着异育银鲫(Carassius auratus gibelio)养殖密度的增大,其主要养殖区爆发了以鳃部出血为典型特征的疾病,经确诊为鲫造血器官坏死症(Crucian Carp Hematopoietic Necrosis),引起该病的首要诱因为鲤疱疹病毒Ⅱ型(CyHV-2),给异育银鲫养殖造成了巨大的经济损失。溶氧是水生生物生存的必要条件之一,溶氧条件的改变直接影响水生生物的各项生理机能,在溶氧胁迫状态下,水生生物会表现出应激及免疫力下降,从而导致疾病的发生。为了探究溶氧浓度对异育银鲫感染CyHV-2及发生鲫造血器官坏死症的影响,将健康的异育银鲫与已感染CyHV-2的病鱼混合饲养,结合鲫的最低生存氧浓度,设定溶氧浓度分别为7、6、5、4、3 mg/L,不同溶氧浓度均设立对照组,在相同的饲养条件下,通过控制不同溶氧浓度,测定CyHV-2感染数及死亡数,分析不同溶氧浓度条件下鲫CyHV-2感染率及鲫造血器官坏死症的发病率。结果表明,随着溶氧浓度的降低,CyHV-2的感染率和死亡率都随之升高,当溶氧浓度为7、6、5、4 mg/L时,对照组均未出现感染及死亡状况;当溶氧浓度为 3 mg/L时,实验组感染率为100%并且对照组也出现了死亡,充分肯定了溶氧胁迫会增加异育银鲫对CyHV-2的感染率及死亡率。研究结果对异育银鲫的大规模养殖及造血器官坏死症防控具有重要的意义。
英文摘要:
      In recent years, as breeding density has increased, Carassius auratus gibelio hemorrhagic disease has broken out on a large scale in the main breeding area of Jiangsu Province and caused severe economic losses in aquaculture. The disease was diagnosed as Crucian Carp Hematopoietic Necrosis and characterized by bleeding from gills and muscles. Research has shown that Cyprinid herpesvirus Ⅱ(CyHV-2) is the primary virus responsible for the disease and identifying the factors that lead to outbreaks is important. Dissolved oxygen (DO) is essential for aquatic life and low DO stress could suppress the immune response. We hypothesized that DO was an important factor in the occurrence of Crucian Carp Hematopoietic Necrosis and the effect of varying DO on the infection rate and mortality of C. auratus gibelio infected with CyHV-2 was investigated. Healthy C. auratus gibelio (body weight, 550-620 g; length, 22-26 cm) were acclimated in an indoor circulating aquaculture system for a week. The DO stress experiment was carried out in aquaria (80 cm×65 cm×60 cm ) at five DO treatment levels: 7mg/L, 6 mg/L, 5 mg/L, 4 mg/L and 3 mg/L, based on the minimum DO requirement of crucian carp. Each treatment group was composed of twenty healthy C. auratus gibelio and two C. auratus gibelio infected with CyHV-2 and there was a control group for each DO treatment with 22 healthy test fish. All fish were cultured at the same temperature (27.0±0.5℃and pH (7.2±0.2). The test lasted 14 days and the number of dead fish and fish presenting symptoms of infection were recorded each day. After the test, all fish were tested for CyHV-2, including fish that died during the experiment. The infection rate and mortality of C. auratus gibelio increased as DO decreased. No infection occurred in any of the control groups, but there was mortality in the lowest DO control group (3 mg/L), due to hypoxia. In conclusion, DO stress increases the infection rate and mortality of C. auratus gibelio exposed to CyHV-2. The results will be useful in preventing and controlling Crucian Carp Hematopoietic Necrosis and support intensive breeding of C. auratus gibelio.
赵彦华,孙梦玲,万金娟,夏爱军,薛 晖.2018.溶氧胁迫对异育银鲫感染鲫造血器官坏死症的影响[J].水生态学杂志,39(6):95-99.
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