UV-B对紫球藻生长抑制及生理特性的影响
Effects of UV-B Radiation on the Growth and Physiological Properties of Porphyridium purpureum
投稿时间:2017-03-15  修订日期:2018-11-17
中文关键词:UV-B  紫球藻  抑制作用  生理特性
英文关键词:UV-B  Porphyridium purpureum  inhibition  physiological characteristics
基金项目:国家自然科学基金项目(41373023;91544229-02);江苏省“333高层次人才培养工程”与江苏省大学生实践创新训练计划项目(201510300048;201610300038)
作者单位E-mail
王静 南京信息工程大学环境科学与工程学院江苏 南京 210044 410876304@qq.com 
郭照冰 南京信息工程大学环境科学与工程学院江苏 南京 210044 guozbnuist@163.com 
王瑾瑾 南京信息工程大学环境科学与工程学院江苏 南京 210044 617647460@qq.com 
韩珣 南京信息工程大学环境科学与工程学院江苏 南京 210044 312011553@qq.com 
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中文摘要:
      由于氟氯烷烃化合物(CFCs)的污染,日趋严重的臭氧层(Atmospheric ozone)侵蚀的问题,使得到达地面的紫外线B(UV-B:280-315nm)波段的辐射不断增强,通过影响海洋微藻的初级生产率从而导致了海洋生态化境的变化。为了探究UV-B辐照对紫球藻(Porphyridium purpureum)细胞的作用机理,具体量化胁迫抑制剂量,通过考察紫球藻细胞生长对不同辐照剂量、pH值及培养基的响应,结合紫球藻细胞叶绿素a(Chl-a)、藻红蛋白(PE)、多糖、多不饱和脂肪酸-二十碳五烯酸(EPA)、超氧化歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)及丙二醛(MDA)生成量的变化情况,对比分析了其对紫球藻细胞内含物及细胞代谢活性的持续性影响。UV-B辐照通过固定强度为0.35 W/m2的紫外平行光束仪,调节照射时间以得到实验设计辐射剂量0、75、150、200、250、300、400、500 mJ/cm2,对应照射时间分别为0、36、72、96、120、144、192 min。结果表明,高剂量的UV-B辐照对紫球藻细胞生长有更为明显的抑制作用,辐照剂量大于300 mJ/cm2时会直接导致藻细胞群体的大量死亡,抑制并破坏紫球藻细胞内光合色素和藻红蛋白的合成。碱性环境和培养基本身对UV-B辐照抑制紫球藻细胞的生长有一定阻碍。紫球藻细胞内抗氧化酶(SOD、POD、CAT)活性随UV-B辐照剂量的增加先升高后降低,MDA则持续增加。EPA的生成量随UV-B辐照剂量的增大而减少,低剂量的UV-B辐照(75-150 mJ/cm2)可促进紫球藻胞外多糖的合成。研究结果可为紫球藻生物活性物质及其衍生物的生产应用提供理论依据,也在一定程度上确立了利用UV-B辐照技术控制紫球藻藻华的工艺条件。
英文摘要:
      The depletion of atmospheric ozone by chlorofluorocarbons (CFCs) has increased ground level radiation in the UV-B band (280-315nm). The increased UV-B radiation has affected primary production in microalgae and altered marine ecology. The purpose of this study was to explore the inhibition mechanism of UV-B radiation on Porphyridium purpureum growth at different UV-B intensities. The effects of UV-B dose, solution pH, and growth media on cell composition and metabolism of P. purpureum were investigated by monitoring cell content of chlorophyll-a (Chl-a), phycoerythrin (PE), extracellular polysaccharides, eicosapentaenoic acid (EPA), superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and malondialdehyde (MDA). The UV radiation source was a collimated UV beam apparatus with a fixed radiant intensity of 0.35 W/m2. The experimental UV treatment doses were achieved by controlling the irradiation time at the fixed intensity, with irradiation times of 0, 36, 72, 96, 120, 144,192 and 240 min producing corresponding UV doses of 0, 75, 150, 200, 250, 300, 400 and 500 mJ/cm2. The highest UV-B dose had an obvious inhibitory effect on P. purpureum development. Algal cells died quickly at UV doses above 300 mJ/cm2, which caused cell damage and inhibited synthesis of chlorophyll and phycoerythrin. Alkaline conditions and higher media nutrient levels reduced growth suppression of P. purpureum by UV-B irradiation to a limited extent. Although MDA rose continuously with increasing UV-B dose, antioxidant enzyme activities (SOD, POD and CAT) initially increased and then decreased with dose. Further, increased UV-B dose decreased cell EPA content and low UV-B doses (75-150 mJ/cm2) increased production of extracellular polysaccharide. The study provides a theoretical foundation for the production of biological active substances by P. purpureum and establishes conditions for controlling algal blooms by UV-B irradiation.
王静,郭照冰,王瑾瑾,韩珣.2018.UV-B对紫球藻生长抑制及生理特性的影响[J].水生态学杂志,39(6):114-120.
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