纤细裸藻对5种抗生素的胁迫响应
Response of Euglena gracilis to Five Kinds of Antibiotic Stress
投稿时间:2017-03-14  修订日期:2017-04-09
中文关键词:抗生素  纤细裸藻  胁迫  响应
英文关键词:antibiotic  Euglena gracilis  stress  response
基金项目:2017-03-14 基金项目:天津市水产产业技术体系创新团队项目(ITTFRS2017005);天津市科技重大专项与工程项目(15ZXBFNC00120);卫星海洋环境动力学国家重点实验室开放基金项目(SOED1419);农业部南海渔业资源开发利用重点实验室开放基金(FREU2015-04);农业部水产品加工重点实验室开放基金(NYGJ201508);天津农学院研究生创新培育项目(2017YPY031)
作者单位E-mail
张文慧 天津市水产生态与养殖重点实验室 天津农学院水产学院天津 300384; 773765349@qq.com 
窦勇 天津市水产生态与养殖重点实验室 天津农学院水产学院天津 300384; 351749424@qq.com 
高金伟 天津市水产生态与养殖重点实验室 天津农学院水产学院天津 300384; 56965198@qq.com 
姜智飞 天津市水产生态与养殖重点实验室 天津农学院水产学院天津 300384; 973918567@qq.com 
贾旭颖 天津市水产生态与养殖重点实验室 天津农学院水产学院天津 300384; 165139810@qq.com 
周文礼 天津市水产生态与养殖重点实验室 天津农学院水产学院天津 300384; 113784778@qq.com 
王永苓 天津海友佳音生物科技股份有限公司天津 300350  
高建忠 天津市泥鳅育种与高效利用技术企业重点实验室天津 301800  
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中文摘要:
      裸藻是一种重要的资源微藻,含有丰富的氨基酸、不饱和脂肪酸、维生素和裸藻糖等营养物质,并且裸藻没有细胞壁,其营养成分相对于其他藻类更容易被人体、水产动物吸收,开展裸藻集约化培养,对优化养殖环境具有十分重要的意义。为了研究裸藻的无菌化养殖,实验生态条件下,研究了不同浓度的5种抗生素(遗传霉素、氯霉素、青霉素、土霉素和链霉素)胁迫对纤细裸藻(Euglena gracilis)细胞密度及叶绿素a水平的影响。结果表明,在遗传霉素、土霉素和链霉素胁迫作用下,裸藻细胞密度显著降低(P<0.05),低于25 μg/mL的遗传霉素即可对裸藻生长与叶绿素a合成产生抑制,200 μg/mL的土霉素和25 μg/mL、50 μg/mL的链霉素对裸藻叶绿素a合成有一定的促进作用;氯霉素和青霉素对裸藻生长有一定的促进效应,200 μg/mL的青霉素可以显著提高纤细裸藻的细胞密度和叶绿素a水平(P<0.05)。实验结果说明纤细裸藻对遗传霉素、土霉素和链霉素的敏感性较强,而氯霉素和青霉素可以作为纤细裸藻无菌系建立的备选抗生素,研究结果可为纤细裸藻的基因工程选择标记和无菌培养体系的建立提供参考。
英文摘要:
      As an excellent nutritional resource, the microalgae Euglena gracilis is rich in complex nutrients, including amino acids, unsaturated fatty acids and vitamins. Additionally, the nutrients derived from E. gracilis are more easily absorbed by humans and aquatic animals than from other algae because it has no cell walls. Therefore, it is important to develop intensive cultivation methods and optimize the breeding environment for E. gracilis. In this study, we investigated the variation of cell density and chlorophyll-a (Chl-a) levels of E. gracilis cultured exposed to different concentrations of five antibiotics: Geneticin, Chloramphenicol, Penicillin, Oxytetracycline and Streptomycin, to explore sterile culture methods and provide basic data for establishing a sterile system for E. gracilis. The experiment was carried out under laboratory conditions. E. gracilis was cultured to the logarithmic phase for the test, with a cell density of 5×105 cells/mL under the following conditions: temperature of (25±1) ℃, illumination of 60 μmol/(m2?s) and a light-dark cycle of 12:12. Antibiotics were added to culture solutions to give Chloramphenicol, Penicillin, Oxytetracycline and Streptomycin concentrations of 25, 50, 75, 100, 200 μg/mL and Geneticin concentrations of 25, 50, 75, 100, 125 μg/mL and each treatment was run in triplicate. After treatment, culture solutions were sampled once a day for determination of cell density and Chl-a content. Geneticin, Oxytetracycline, and Streptomycin significantly decreased cell density (P<0.05) and Geneticin, at concentrations under 25 μg/mL, significantly inhibited growth and Chl-a synthesis. Oxytetracycline (200 μg/mL) and Streptomycin (25–50 μg/mL) increased Chl-a content, while Chloramphenicol and Penicillin promoted growth. Penicillin (200 μg/mL) significantly enhanced cell density and Chl-a levels (P<0.05). Results indicate that E. gracilis is sensitive to Geneticin, Oxytetracycline, and Streptomycin and that Chloramphenicol and Penicillin can be used as antibiotics for axenic culture of E. gracilis. The results will also serve as reference for identifying a selective marker of E. gracilis genetic engineering and the axenic culture of microalgae.
张文慧,窦勇,高金伟,姜智飞,贾旭颖,周文礼,王永苓,高建忠.2017.纤细裸藻对5种抗生素的胁迫响应[J].水生态学杂志,38(6):48-56.
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