池塘养殖水体中土著反硝化细菌的分离与初步鉴定
Isolation and Preliminary Identification of Native Denitrification Bacteria from an Aquaculture Pond
投稿时间:2017-03-10  修订日期:2018-09-12
中文关键词:池塘  养殖水体  反硝化菌  分离  鉴定
英文关键词:aquaculture pond  aquaculture wastewater  native denitrifying bacteria  isolation  identification
基金项目:山西省水利推广项目(2130317)。
作者单位E-mail
张元 山西省水产育种养殖科学实验中心山西 太原 030002 252259565@qq.com 
摘要点击次数: 140
全文下载次数: 221
中文摘要:
      池塘养殖水体水质恶化问题日益突出,尽管已有各种商品化微生态制剂,但水质净化修复效果始终未得到有效提高。通过采用定向分离、筛选、扩繁来自原生境的土著有益菌的方法进行生物修复,从而提高养殖废水的净化修复效果;该方法具有针对性强、效果佳、安全性高、成本低以及持续活性时间长、二次污染等优点。2016年9月,从山西省运城黄河滩涂水产养殖主产区养殖中后期的池塘水体中定向分离筛选得到一株土著反硝化细菌,命名为YJ-1。使用奈氏试剂和格利斯试剂对其反硝化能力进行检测,结果显示,奈氏试剂显示黄色,格利斯试剂显示绛红色,说明在细菌培养24h后发酵液中产生了少部分的铵离子和较多的亚硝酸盐,菌落具有反硝化功能。经16S rDNA序列扩增与测定,片段长度为1441 bp。根系统发育树分析显示,YJ-1与产酸克雷伯氏杆菌(Klebsiella oxytoca)的同源性最高,YJ-1的分离打破了现有微生态制剂成分大多数为各种商品菌的局势;在水产微生态制剂制备过程中定向加入土著反硝化细菌,可有效降低养殖水体中氨氮与亚硝酸盐含量、减少鱼体发病率;反硝化细菌在水产养殖水质净化、城市污水处理中具有较大的应用前景,本研究为其他土著有益菌的分离、筛选、鉴定及应用提供了可借鉴的思路。
英文摘要:
      Water quality deterioration in aquaculture ponds is a serious problem, and various commercial microbe preparations have emerged, but they have not effectively improved water quality. This study addresses the problem, using directional separation, screening, and propagation of beneficial indigenous bacteria with the objective of improving bio-restoration, purification and remediation of aquaculture wastewater. This approach has the advantages of beneficial effect, high safety, low cost, extended activity and no secondary pollution. In this study, one native denitrifying bacteria, named YJ-1, was isolated and screened from pond water during the mid-late stage (September) of the 2016 aquaculture season. The pond was located in the Yellow River tidal flat of Yuncheng City, the main fish production area of Shanxi Province. During denitrification capacity testing, ammonia was determined with Nessler reagent (yellow) and nitrite was determined with Gries reagent (red). After 24 h of fermentation, ammonia was at a low concentration and nitrite was produced, indicating denitrification. The results of 16S rDNA amplification and sequencing indicated a fragment length of 1441bp. Unrooted phylogenetic tree analysis showed that YJ-1 and Klebsiella oxytoca had the highest homology. Successful isolation of YJ-1 interrupts the trend of microbe preparations coming from commercial sources. If native denitrifying bacteria were added to preparations, ammonia and nitrite nitrogen in aquaculture water could be effectively reduced. Denitrifying bacteria show good potential for application in aquaculture wastewater purification and urban sewage treatment. This study also provides a promising method for isolation, screening, identification and application of other beneficial native bacteria.
张元.2018.池塘养殖水体中土著反硝化细菌的分离与初步鉴定[J].水生态学杂志,39(5):111-115.
查看全文  查看/发表评论  下载PDF阅读器
Copyright © 2008 《水生态学杂志》编辑部 地址:湖北省武汉市雄楚大街578号 
邮编:430079 电话:027-82926630 E-mail: sstx@mail.ihe.ac.cn  京ICP备09084417号