| The variation in induction velocities among different fish species, and the related driving factors, was investigated to provide base data to support fish passage design. Here, the induction velocity of five fish species (Cyprinus carpio, Carassius auratus, Hypophthalmichthys molitrix, Aristichthys nobilis and Culter mongolicus mongolicus) were measured and compared. The objective was to supplement data on the rheotaxis characteristics of the species investigated. Hypophthalmichthys molitrix and Aristichthys nobilis were collected for testing from a fishing ground, located in Hanzhong City, Shannxi Province, and the Cyprinus carpio, Carassius auratus and Culter mongolicus mongolicus were wild and collected from Hanjiang River. Body lengths(cm) of the test fish were, respectively (32.73±3.7), (30.47±1.7), (24.46±5.9), (13.66±4.7), and (23.51±3.1). Before testing, all fish were acclimated for 3 hr in the aquarium with water continuously recycled and aerated. The experiment was carried out in custom made test chamber with a motor-driven propeller. Test water was taken from a mountain stream and testing was carried out at (21?1) oC,with a DO of (6.5?0.5) mg/L. Flow velocity in the test chamber was controlled by calibrating the velocity to motor speed (Hz). The flow velocity that induced swimming (induction velocity) was then determined by observing the fish as flow velocity in the test chamber was increased. Bivariate correlation analysis was used to analyze the relationship between the induction velocity and body length for each of the five fish species, and the independent sample Kruskal-Wallis test was used to analyze the difference of induction velocity between the five fish species. Results show that the absolute induction velocities (cm?s-1) of Cyprinus carpio, Carassius auratus, Hypophthalmichthys molitrix, Aristichthys nobilis and Culter mongolicus mongolicus were, respectively, (3.55±2.43), (2.408±2.00), (4.605±2.575), (2.033±1.019) and (1.843±1.205) m?s-1, and the relative induction velocities(BL?s-1) were (0.147±0.097), (0.182±0.166), (0.142±0.07), (0.067±0.034), and (0.08±0.054). The absolute induction velocity for the five fish species did not correlate with body length, so relative induction velocity is a more precise indicator of the response of fish to change in water velocity. The induction velocities of the five species were significantly different (P<0.01). Of the five fish species, Aristichthys nobilis was the most sensitive species to the variation of water flow, followed by Culter mongolicus mongolicus, Hypophthalmichthys molitrix, Cyprinus carpio and Carassius auratus, consistent with expected habitat adaptations. There was no significant difference in the absolute induction velocity between Aristichthys nobilis and Culter mongolicus mongolicus or between Hypophthalmichthys molitrix, Cyprinus carpio and Carassius auratus, but significant differences were observed in the absolute induction velocity among the two groups. The induction velocity is a key parameter for the design of fish passages and, according to our research, the different induction velocities of target fishes should be considered in fish passage design.