肉桂醛纳米乳的制备及其抑菌效果研究
Preparation of Cinnamaldehyde Nanoemulsion (CNE) and Its Antibacterial Effects
投稿时间:2016-06-04  修订日期:2018-04-12
中文关键词:肉桂醛  纳米乳  最小抑菌浓度
英文关键词:Cinnamaldehyde  Nanoemulsion  Minimal Inhibitory Concentration
基金项目:天津市应用基础与前沿技术研究计划(重点项目)(15JCZDJC33600);天津市“现代水产生态健康养殖”创新团队基金项目(TD12-5018);天津市高等学校大学生创新创业训练计划项目(201510061044)
作者单位E-mail
王庆奎 天津市水产生态及养殖重点实验室
天津农学院水产学院 
wangqk@tjau.edu.cn 
姚维林 天津市水产生态及养殖重点实验室
天津农学院水产学院 
1174336823@qq.com 
陆 颖 天津市水产生态及养殖重点实验室
天津农学院水产学院 
luying.magic@foxmail.com 
石洪玥 天津市水产生态及养殖重点实验室
天津农学院水产学院 
61350535@qq.com 
卢正义 天津市晨辉饲料有限公司 37918538@qq.com 
孙学亮 天津市水产生态及养殖重点实验室
天津农学院水产学院 
403986636@qq.com 
毕相东 天津市水产生态及养殖重点实验室
天津农学院水产学院 
19144396@qq.com 
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中文摘要:
      为评价肉桂醛纳米乳对水产动物致病菌的抑菌效果,以吐温-80为表面活性剂、乙醇为助表面活性剂、乙酸乙酯为油相和蒸馏水按比例配制成空白纳米乳,加入肉桂醛得肉桂醛纳米乳,并评价了肉桂醛纳米乳的部分特性。比较了肉桂醛纳米乳对迟缓爱德华氏菌(Edwardsiella tarda)、嗜水气单胞菌(Aeromonas hydrophila)、温和气单胞菌(Aeromonas sobria)、鳗败血假单胞菌(Pseudomonas anquilliseptica)4种常见水产动物致病菌的抑菌效果。结果表明,肉桂醛纳米乳中吐温-80、乙醇、乙酸乙酯、蒸馏水和肉桂醛质量比为10 : 5 : 11 : 73 : 1,空白纳米乳对肉桂醛的最大载药量为20.11 mg/mL,肉桂醛纳米乳为水包油型淡黄色澄清透明液体,粒径范围5~20 nm,平均粒径10.74 nm;与同浓度肉桂醛溶液相比,肉桂醛纳米乳具有更强的抑菌活性,在最大载药量时,对嗜水气单胞菌(Aeromonas hydrophila)、鳗败血假单胞菌(Pseudomonas anquilliseptica)、迟缓爱德华氏菌(Edwardsiella tarda)、温和气单胞菌(Aeromonas sobria)的抑菌强度分别提高了65.56%、46.99%、41.94%、26.91%。肉桂醛纳米乳对嗜水气单胞菌、温和气单胞菌、鳗败血假单胞菌、迟缓爱德华氏菌的最小抑菌浓度(MIC)分别0.31 mg/mL、0.51 mg/mL、0.72 mg/mL、1.00 mg/mL。上述结果表明,肉桂醛纳米乳在水产养殖中具有潜在的应用价值。
英文摘要:
      In order to evaluate the antibacterial effect of the cinnamaldehyde nanoemulsion (CNE) against the pathogenic bacteria of aquatic animals, the tween-80 was adopted as surfactant, ethanol as cosurfactant, ethyl acetate as oil phase, along with distilled water, to prepare blank nanoemulsion. Cinnamaldehyde was mixed into the blank nanoemulsion to form the cinnamaldehyde nanoemulsion (CNE). Some physical characteristics of the CNE were evaluated. The antibacterial activity of the CNE against four pathogenic bacteria of aquatic animals, Edwardsiella tarda, Aeromonas hydrophila, Aeromonas sobria, and Pseudomonas anquilliseptica, was compared. The results show that the optimum mass ratio of tween-80 : ethanol : ethyl acetate : distilled water : cinnamaldehyde was 10 : 5 : 11 : 73 : 1. The maximum cinnamaldehyde loading of blank nanoemulsion is 20.11 mg/mL. The oil-in-water CNE is light yellow, clear and transparent, with particle size range from 5 nm to 20 nm, average 10.74 nm. Compared with the same concentration of cinnamaldehyde, the CNE exhibits stronger antimicrobial activity. At 20.11 mg/mL, the antibacterial effect of CNE against A. hydrophila, P. anquilliseptica, E. tarda and A. sobria was enhanced 65.56%, 46.99%, 41.94%, 26.91%, respectively. The Minimal Inhibitory Concentration of CNE against A. hydrophila, A. sobria, P. anquilliseptica and E. tarda was 0.3, 0.5, 0.7 and 1 mg/mL, respectively. These findings demonstrated that CNE owns potential application value in aquaculture.
王庆奎,姚维林,陆 颖,石洪玥,卢正义,孙学亮,毕相东.2018.肉桂醛纳米乳的制备及其抑菌效果研究[J].水生态学杂志,39(2):95-100.
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