肉桂醛纳米乳的制备及其抑菌效果研究
Preparation of Cinnamaldehyde Nanoemulsion (CNE) and Its Antibacterial Effects
投稿时间:2016-06-04  修订日期:2018-04-12
中文关键词:肉桂醛  纳米乳  最小抑菌浓度
英文关键词:cinnamaldehyde  nanoemulsion  minimal inhibitory concentration (MIC)
基金项目:天津市应用基础与前沿技术研究计划(重点项目)(15JCZDJC33600;15JCZDJC34000);天津市“现代水产生态健康养殖”创新团队基金项目(TD12-5018);天津市水产产业技术体系创新团队基金项目(ITTFRS2017004;ITTFRS2017003);天津市科技支撑重点项目(16JCTPJC44600);国家自然科学基金青年项目(31402313);天津市高等学校大学生创新创业训练计划项目(201510061044)
作者单位E-mail
王庆奎 天津市水产生态及养殖重点实验室天津 300384天津农学院水产学院天津 300384 wangqk@tjau.edu.cn 
陆颖 天津市水产生态及养殖重点实验室天津 300384天津农学院水产学院天津 300384 luying.magic@foxmail.com 
姚维林 天津市水产生态及养殖重点实验室天津 300384天津农学院水产学院天津 300384 1174336823@qq.com 
石洪玥 天津市水产生态及养殖重点实验室天津 300384天津农学院水产学院天津 300384 61350535@qq.com 
卢正义 天津市晨辉饲料有限公司天津301800 37918538@qq.com 
孙学亮 天津市水产生态及养殖重点实验室天津 300384天津农学院水产学院天津 300384 403986636@qq.com 
毕相东 天津市水产生态及养殖重点实验室天津 300384天津农学院水产学院天津 300384 19144396@qq.com 
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中文摘要:
      肉桂醛(Cinnamaldehyde)是从肉桂(Cinnamomum cassia Presl)中提取的脂溶性化合物,具有很强的杀菌活性。纳米乳能使脂溶性物质更易分散于水中。为评价肉桂醛纳米乳对水产动物致病菌的抑菌效果,以吐温-80为表面活性剂、乙醇为助表面活性剂、乙酸乙酯为油相和蒸馏水按比例配制成空白纳米乳,加入肉桂醛制备得肉桂醛纳米乳,并评价了肉桂醛纳米乳的部分特性;比较了肉桂醛纳米乳对迟缓爱德华氏菌(Edwardsiella tarda)、嗜水气单胞菌(Aeromonas hydrophila)、温和气单胞菌(Aeromonas sobria)、鳗败血假单胞菌(Pseudomonas anguilliseptica)4种常见水产动物致病菌的抑菌效果。结果显示,肉桂醛纳米乳中吐温-80、乙醇、乙酸乙酯、蒸馏水和肉桂醛质量比为10 : 5 : 11 : 73 : 1;该纳米乳对肉桂醛的最大载药量为20.11 mg/mL;肉桂醛纳米乳为水包油型淡黄色澄清透明液体,粒径5~20 nm,平均粒径10.74 nm;与同浓度肉桂醛溶液相比,肉桂醛纳米乳具有更强的抑菌活性,在最大载药量时,对嗜水气单胞菌、鳗败血假单胞菌、迟缓爱德华氏菌、温和气单胞菌的抑菌强度分别提高了65.56%、46.99%、41.94%、26.91%。肉桂醛纳米乳对嗜水气单胞菌、温和气单胞菌、鳗败血假单胞菌、迟缓爱德华氏菌的最小抑菌浓度(MIC)分别0.31、0.51、0.72、1.00 mg/mL。上述研究表明,肉桂醛纳米乳在水产养殖中具有潜在的应用价值
英文摘要:
      Cinnamaldehyde is a lipid soluble compound extracted from Cinnamomum cassia Presl and widely used in livestock and poultry fodder. It has strong bactericidal activity and is used for animal food preservation because it is metabolized quickly and leaves no residue in animals, does not pollute the breeding environment and pathogenic bacteria do not readily develop resistance to cinnamaldehyde. Because cinnamaldehyde is not water soluble, application in water is of limited effectiveness. However, lipid soluble substances can be effectively dispersed in water as a nanoemulsion. In this study, a cinnamaldehyde nanoemulsion (CNE) was prepared, the physical characteristics described and the antibacterial effects evaluated with four common pathogenic bacteria of aquatic animals: Edwardsiella tarda, Aeromonas hydrophila, Aeromonas sobria and Pseudomonas anguilliseptica. The antibacterial activity of the CNE against the four pathogenic bacteria was compared with that of aqueous suspensions of cinnamaldehyde (CAS) at the same concentration. The results provide a reference for the use of CNE in aquaculture. To prepare the CNE, polysorbate-80 was used as the surfactant, ethanol as cosurfactant and ethyl acetate as the oil phase. Cinnamaldehyde was then mixed with the nanoemulsion to form CNE and an equal volume of distilled water was used to prepare the nanoemulsion blank. The optimal mass ratio for CNE was polysorbate-80:ethanol:ethyl acetate:distilled water:cinnamaldehyde was 10:5:11:73:1. The resulting CNE was light yellow and clear, with a particle size range of 5 - 20 nm and an average value of 10.74 nm. The maximum loading capacity of cinnamaldehyde as CNE was 20.11 mg/mL and at this level, CNE and CAS both clearly displayed antibacterial activity. The antibacterial activity of CNE against the four common pathogenic bacteria followed the order P. anguilliseptica> A. hydrophila>E. tarda > A. sobria, while the activity of CA followed the order P. anguilliseptica >E. tarda >A. sobria >A. hydrophila. The antibacterial effect of CNE against A. hydrophila, P. anguilliseptica, E. tarda and A. sobria was enhanced, respectively, by 65.56%, 46.99%, 41.94%, and 26.91%, compared to CAS at the same dose level. The minimal inhibitory concentrations (MIC) of CNE against A. hydrophila, A. sobria, P. anguilliseptica and E. tarda were, respectively, 0.31, 0.51, 0.71 and 1.00 mg/mL and the antibacterial activity of CNE at the MIC were 50.00%, 38.27%, 34.55% and 38.27% higher than CA. These findings demonstrate that CNE has high potential value for use in aquaculture.
王庆奎,陆颖,姚维林,石洪玥,卢正义,孙学亮,毕相东.2018.肉桂醛纳米乳的制备及其抑菌效果研究[J].水生态学杂志,39(2):95-100.
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