| 圆口铜鱼维氏气单孢菌的分离鉴定及药敏试验 |
| Isolation, Identification and Drug Susceptibility Test of Aeromonas veronii from Coreius guichenoti |
| 投稿时间：2016-05-06 修订日期：2016-07-28 |
| DOI：10.15928/j.1674-3075.2016.04.015 |
| 中文关键词:圆口铜鱼 维氏气单胞菌 生化鉴定 16S rDNA序列 药敏试验 |
| 英文关键词:Coreius guichenoti Aeromonas veronii biochemical identification 16S rDNA sequence susceptibility test |
| 基金项目:圆口铜鱼人工繁殖技术研究(2) ( JGJ /0242013) |
| 作者 | 单位 | E-mail | | 曹恒源* | 贵州大学动物科学学院，贵阳 550025；水利部中国科学院水工程生态研究所, 水利部水工程生态效应与生态修复重点试验室, 武汉 430079 | caohengyuan123@126.com | | 乔晔 | 水利部中国科学院水工程生态研究所, 水利部水工程生态效应与生态修复重点试验室, 武汉 430079 | | | 陈小娟 | 水利部中国科学院水工程生态研究所, 水利部水工程生态效应与生态修复重点试验室, 武汉 430079 | | | 潘磊 | 水利部中国科学院水工程生态研究所, 水利部水工程生态效应与生态修复重点试验室, 武汉 430079 | | | 谢建军 | 水利部中国科学院水工程生态研究所, 水利部水工程生态效应与生态修复重点试验室, 武汉 430079 | | | 董纯 | 水利部中国科学院水工程生态研究所, 水利部水工程生态效应与生态修复重点试验室, 武汉 430079 | | | 安苗 | 贵州大学动物科学学院，贵阳 550025； | | | 王小明 | 贵州大学动物科学学院，贵阳 550025； | | | 杨少荣 | 中国三峡建设管理有限公司， 成都 610041 | |
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| 中文摘要: |
| 从濒死圆口铜鱼的脾和肾病灶部位分离优势菌，进行纯化分离并开展生理生化鉴定、16S rDNA序列分析以及常用抗生素药物的药敏性筛选，以期丰富圆口铜鱼病害防治的基础资料。分离出的1株优势菌在BHI固体培养基上长出表面光滑的灰白色圆形菌落，边缘整齐、不透明、稍隆起； 16S rDNA序列长度为1399 bp，与维氏气单孢菌的同源性为100%，与维氏气单孢菌（KT964297.1；KJ650080.1；KM362731.1）聚为一支，置信度99。结合菌落形态特征和生理生化特征，鉴定菌株为维氏气单孢菌。该菌株对氟苯尼考等7种药物敏感，对利福平中度敏感，对新霉素等9种药物耐药。 |
| 英文摘要: |
| The early life resource of Coreius guichenoti is decreasing significantly year by year in its primary habitat, the middle Jiangsha River, and this is arousing concern about the need for conservation. Fish disease is the primary problem for domestication and propagation of Coreius guichenoti. In July, 2015, an unknown bacterial disease broke out in the domesticated Coreius guichenoti in Wuhan, resulting in the death of over ten fish in a short time. To identify the pathogen and explore prevention strategies, a dominant strain, labeled YT01, was isolated from the spleen and kidney of moribund Coreius guichenoti. Biochemical analysis, 16S rDNA sequencing and an antibiotic susceptibility test were carried out and the data generated will support efforts to control and prevent disease in Coreius guichenoti. The isolated YT01 strain, grown on BHI solid media developed round gray-white colonies with smooth surfaces, neat, opaque and slightly raised. The 16Sr DNA sequence of YT01 strain was 1399 bp long and 100% homologous to Aeromonas veronii. A phylogenic tree was constructed based on the 16S rDNA sequence of the YT01 strain and 16S rDNA sequences from another 25 Aeromonas strains obtained from the NCBI GenBank database. The sequence of the YT01 strain clustered into a clade with Aeromonas veronii (KT964297.1; KJ650080.1; KM362731.1), with a confidence level of 99%. Combined with the morphological characteristics and physiological and biochemical characteristics of the colony, the YT01 strain was conclusively identified as Aeromonas veronii. Antibiotic susceptibility of the YT01 strain was tested using 17 common antibiotics. Results show that the YT01 strain is highly sensitive to 7 antibiotics, including florfenicol, moderately sensitive to rifampin and strongly resistant to 9 antibiotics, including neomycin. |
| 曹恒源,乔晔,陈小娟,潘磊,谢建军,董纯,安苗,王小明,杨少荣.2016.圆口铜鱼维氏气单孢菌的分离鉴定及药敏试验[J].水生态学杂志,37(4):95-100. |
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