| 白梭吻鲈3个群体的遗传结构比较 |
| Genetic Structure Analysis of Three Pikeperch (Sander lucioperca) Populations by Microsatellite Markers |
| 投稿时间：2016-01-26 修订日期：2017-01-11 |
| DOI：10.15928/j.1674-3075.2017.01.013 |
| 中文关键词:白梭吻鲈 微卫星 遗传结构 |
| 英文关键词:Sander lucioperca microsatellite markers genetic structure |
| 基金项目:江苏省科技厅项目（BY2015039-10）；常熟市科技计划项目（CN201408) |
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| 中文摘要: |
| 为了加强良种选育工作，切实做好亲本遗传管理，防止近交衰退的发生，利用20个微卫星分子标记对白梭吻鲈(Sander lucioperca)新疆野生群体及山东和苏州2个养殖群体的遗传结构进行检测。结果表明，20个微卫星标记中18个有扩增产物，14个呈现多态性；每个位点的等位基因数为2～6个，平均等位基因数为3.6个，3个群体的平均等位基因数为2.57～3.36，平均观测杂合度为0.5085～0.5621，平均多态性信息含量为0.3931～0.4764，表明3个白梭吻鲈群体的遗传多样性处于中等偏低水平，遗传多样性大小为：新疆群体>苏州群体>山东群体。群体的Fst为0.1798, 表明群体间有一定的遗传分化。在白梭吻鲈人工繁殖与养殖过程中，必须加强亲本遗传结构监测并维持一定数量的亲本规模，以利于其产业的持续发展。 |
| 英文摘要: |
| The pikeperch (Sander lucioperca) is an economically important fresh water fish with desirable characteristics： strong disease-resistance, fast growth, good taste and high protein content. Currently, artificial breeding, seed breeding and culturing of Sander lucioperca is carried out in seven Chinese provinces since artificial breeding was first accomplished in 1993. To prevent inbreeding and lay the foundation for genetic breeding of this species, 20 microsatellite markers were used to investigate the genetic structure of three pikeperch populations： a wild population from Xinjiang and two cultured populations from Shandong and Jiangsu. The wild Sander lucioperca were obtained from Ulungur Lake in Fuhai County, Xinjiang, and the two cultured populations were obtained from aquaculture farms in Linyi, Shandong and Suzhou, Jiangsu, with 32 specimens from each population. From among the 20 microsatellite primers, 18 loci were successfully amplified with 14 polymorphic loci for the three populations. Genetic diversity in the three populations was in the medium to low range, based on the following criteria： number of alleles per locus (2-6, avg=3.6), average number of alleles (2.57-3.36), average observed heterozygosity (Ho, 0.5085-0.5621), average expected heterozygosity (He, 0.4715-0.5467) and average polymorphic information content (PIC, 0.3931-0.4764). The order of genetic diversity in the three populations is Xinjiang>Suzhou> Shandong. The genetic differentiation coefficient (Fst) of the three populations was 0.1798. The largest genetic distance was between Xinjiang population and Shandong population with the genetic distance of 0.4691, with a smaller difference, 0.2936, between the Shandong and Suzhou populations. UPGMA cluster analysis of the three pikeperch populations shows that the Shandong and Suzhou populations first clustered into one group and then clustered with the Xinjiang population. Our research suggests that monitoring the genetic background and maintaining large parent populations are necessary to ensure genetic diversity in artificial Sander lucioperca populations and promote the sustainable development of Sander lucioperca aquaculture. |
| 韩晓飞,凌去非,李彩娟,许郑超,王国成.2017.白梭吻鲈3个群体的遗传结构比较[J].水生态学杂志,38(1):80-85. |
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