| 基于15N稳定同位素技术的斜生栅藻对硝氮和氨氮吸收研究 |
| The study on uptake of nitrate and ammonium by Scendesmus obliquus based on 15N stable isotope technique |
| 投稿时间：2011-01-20 修订日期：2011-04-07 |
| DOI： |
| 中文关键词:15N稳定同位素 斜生栅藻 硝氮 氨氮 |
| 英文关键词:15N stable isotope Scendesmus obliquus Nitrate Ammonium |
| 基金项目:国家自然科学基金（U0733007、U1033602和41073057）资助 |
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| 中文摘要: |
| 硝氮(NO3--N)和氨氮(NH4+-N)是水体中无机氮的主要形态。利用15N稳定同位素技术研究了斜生栅藻(Scendesmus obliquus)对NO3--N和NH4+-N的吸收特征。结果显示,在相同浓度条件下,斜生栅藻对NH4+-N的吸收速率显著高于对NO3--N的吸收率,在180min的试验中,对15NH4+-N的吸收速率为0.62～1.15μmol/(g•min);对15NO3--N的吸收速率为0.08～0.15μmol/(g•min)。在NO3--N和NH4+-N2种形态氮源同时存在的混合组中,斜生栅藻对NO3--N的吸收速率[0.12～1.00μmol/(g•min)]显著低于NO3--N作为唯一氮源的单一组[0.78～1.23μmol/(g•min)],表明NH4+-N的存在对藻类吸收NO3--N有抑制作用。在14NO3--N和15NO3--N同时存在时,斜生栅藻优先吸收14NO3--N,产生同位素分馏效应,但不同形态氮对藻类氮吸收的影响远远大于同位素的影响。 |
| 英文摘要: |
| Nitrate(N0-3 -N) and ammonium(NH+4-N)nitrogen are the main forms of inorganic nitrogen in water. This paper studied the uptake characteristics of N0-3 -N and NH+4-N by Scendesmus obliquus based on 15N stable isotope technique. The result showed that the NH+4-N uptake rate was significantly higher than N0-3 -N uptake rate at the same nitrogen concentration, 15NH+4-N uptake rate was 0. 62-1.15μmol /(g•min)while 15N0-3 -N uptake rate was 0. 08-0. 15μmol/(g•min) in this 3 h experiment. The N0-3 -N uptake rate in the mixed nitrogen group[0.12-1.00μmol/(g•min)〕in which both N0-3 -N and NH+4-N presented was significantly lower than that in the single nitrogen group[0. 78-1.23 μmol/ ( g•min〕in which only N0-3 -N presented，indicating that the presence of NH+4-N inhibits the uptake of N0-3 -N. The result also showed that when 15N0-3 -N and 14NH+4-N existed simultaneously，Sceudesmus obliquus took up 14N0-3 -N preferentially which produces isotope fractionation. However, the effect of different forms of nitrogen source is far greater than the effect of isotope fractionation. |
| 包苑榆,钟萍,韦桂峰,郭海森,刘正文.2011.基于15N稳定同位素技术的斜生栅藻对硝氮和氨氮吸收研究[J].水生态学杂志,32(3):16-20. |
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